Test Overview
Test Methodology

Immunofluorescence (IFA), Western Blot

Test Usage

Antineuronal Nuclear Antibody (Anti-Hu) is found in 5-10% of patients with small cell carcinoma of the lung. Anti-Hu is associated with subacute syndrome of encephalomyeloradiculopathy, sensory neuropathy, and autoimmune neuropathy, predominantly affecting the gastrointestinal tract.

Reference Range *

Negative; Western blot= Negative; Hu titer <1:40

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday, Wednesday, Friday
Analytic Time

5 - 9 days

Soft Order Code
MiChart Code
  • Paraneoplastic Autoantibody, Antineuronal Nuclear Antibody 1
  • Antineuronal Nuclear Antibody (ANNA) Type 1
  • Sensory Neuropathy Antibody
  • Anti-Hu
  • Neuronal Nuclear Antibody, Type 1
  • ANNA-1
Reference Laboratory
Quest Valencia 37053
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in an SST or red top tube. Centrifuge, aliquot serum into a plastic vial and refrigerate.

Normal Volume
0.5 mL serum
Minimum Volume
0.2 mL serum
Additional Information

If the Hu Antibody screen by IFA is positive, Hu Antibody by Western Blot will be performed at an additional charge. If the Hu Antibody by Western Blot is positive, Hu Antibody Titer will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Quest Diagnostics Nichols Institute of Valencia.

CPT Code
Reflex CPT
Western Blot: 84181, Titer: 86256.