Test Overview
Test Methodology

Real-Time Polymerase Chain Reaction (PCR)

Test Usage

Detection of herpes simplex virus from neonatal surface swab specimens. If either the culture or PCR result are positive, please consult Pediatric Infectious Diseases for guidance on management.

Reference Range *

Negative for HSV1 DNA and HSV2 DNA

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

None

Test Details
Days Set Up
Daily, specimen is set up within an hour of time received in lab.
Analytic Time

3 hours

Soft Order Code
NPHSV
MiChart Code
Neonatal Herpes Simplex Virus DNA, PCR
Laboratory
Microbiology
Section
Microbiology
Specimen Requirements
Collection

Swab the conjunctivae, mouth , nasopharynx and anus (in that order). Place swab into M4-RT transport medium (preferred). M4, M5, M6, UVT, and UTM media are also acceptable. Eswab transport is not acceptable. Refrigerate.

Alternate Specimen
M4, M5, M6, UVT, and UTM media are also acceptable.
Rejection Criteria
Eswab transport is not acceptable. BD ProbeTec transport is not acceptable. Do not use swabs with calcium alginate tip or wooden shafts.
Contraindications
This test should only be ordered on neonates less than 3 days old. For a surface swab from an infant greater than 3 days old, order “HSV DNA by PCR, Qualitative, Swab” and choose “infant surface swab” as the source. For a visible lesion at any age, order “HSV DNA by PCR, Qualitative, Swab” and indicate the site of the lesion.
Normal Volume
1 swab
Minimum Volume
1 swab
Storage Temperature
Refrigerate
Billing
CPT Code
87529 x2
Fee Code
LA003, LA004
LOINC
20444-6
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