Molecular Typing (Polymerase Chain Reaction with SSOP) and Solid Phase Assays
In general, HLA matched platelets are used to treat those patients who have a poor response to unmatched platelet transfusions. Using SSOP methodology, HLA Class I typing is performed for the -A, -B, and -C loci. Since the presence of HLA antibodies may have an effect on the survival of platelets after transfusion, antibody screening is performed. PRA indicates the degree of reactivity of tested serum in terms of percent of positive reactions when tested against a panel of known HLA phenotypes.
The patient’s HLA Class I typing will be reported. For positive PRA results, the report will define the detected HLA antibody specificities. For negative results, 0% PRA will be reported.
- HLA Antibody Screen Mixed
- HLA Antibody Screening Class I & II
- HLA Class I Low Resolution
- TISSUE TYPING
- HLA REPORT
- New Patient for Plt Support
Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze.
The Class I & II screening assay is performed first and detects the presence/absence of HLA antibodies. If the screening assay is positive, a high specificity and sensitivity single HLA Antigen assay will be performed to identify the specificity of the anti-HLA antibody present in the serum sample from the recipient. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.