Test Overview

Test Methodology

Molecular Typing (Polymerase Chain Reaction with SSOP) and Solid Phase Assays

Test Usage

In general, HLA matched platelets are used to treat those patients who have a poor response to unmatched platelet transfusions. Using SSOP methodology, HLA Class I typing is performed for the -A, -B, and -C loci. Since the presence of HLA antibodies may have an effect on the survival of platelets after transfusion, antibody screening is performed. PRA indicates the degree of reactivity of tested serum in terms of percent of positive reactions when tested against a panel of known HLA phenotypes.

Reference Range*

The patient’s HLA Class I typing will be reported. For positive PRA results, the report will define the detected HLA antibody specificities. For negative results, 0% PRA will be reported.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Monday - Friday, 8:00am - 4:30pm

Analytic Time

5 days

Soft Order Code




HLA Antibody Screen Mixed
HLA Antibody Screening Class I & II
HLA Class I Low Resolution
New Patient for Plt Support

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Specimen Requirements


Yellow Top Tube
ACD Solution A

Normal Volume

10 mL ACD (solution A) whole blood AND 3 mL serum

Minimum Volume

5 mL ACD (solution A) whole blood AND 3 mL serum

Additional Information

The Class I & II screening assay is performed first and detects the presence/absence of HLA antibodies. If the screening assay is positive, a high specificity and sensitivity single HLA Antigen assay will be performed to identify the specificity of the anti-HLA antibody present in the serum sample from the recipient. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

Billing Information

Fee Codes

CPT Code

81372, 86828

Reflex Fee Code

37962, 37963

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