Test Overview
Test Methodology

Molecular Typing (Polymerase Chain Reaction with SSOP) and Solid Phase Assays

Test Usage

In general, HLA matched platelets are used to treat those patients who have a poor response to unmatched platelet transfusions. Using SSOP methodology, HLA Class I typing is performed for the -A, -B, and -C loci. Since the presence of HLA antibodies may have an effect on the survival of platelets after transfusion, antibody screening is performed. PRA indicates the degree of reactivity of tested serum in terms of percent of positive reactions when tested against a panel of known HLA phenotypes.

Reference Range *

The patient’s HLA Class I typing will be reported. For positive PRA results, the report will define the detected HLA antibody specificities. For negative results, 0% PRA will be reported.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
  • HLA Antibody Screen Mixed
  • HLA Antibody Screening Class I & II
  • HLC1L
  • HLA Class I Low Resolution
  • New Patient for Plt Support
Specimen Requirements

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze.

Normal Volume
HLC1L: 10 mL Yellow top (ACD) solution A tube
PRAMO: 5 mL SST tube
Minimum Volume
HLC1L: 5 mL Yellow top (ACD) solution A tube
PRAMO: 3 mL SST tube
Additional Information

The Class I & II screening assay is performed first and detects the presence/absence of HLA antibodies. If the screening assay is positive, a high specificity and sensitivity single HLA Antigen assay will be performed to identify the specificity of the anti-HLA antibody present in the serum sample from the recipient. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
81372, 86828
Fee Code
37921, 37943
Reflex CPT
86832, 86833
Reflex Fee Code
37962, 37963