Test Overview
Test Methodology
HLA Class II Low Resolution HLA typing is achieved via the reverse SSO method that utilizes a suspension array platform using microspheres as a solid support to immobilize oligonucleotide probes. The target DNA is amplified by polymerase chain reactions (PCR) and then hybridized with the bead probe array.
Test Usage
Testing of HLA Class II antigens, DRB1, DRB345, DQB1, DQA1, DPA1, and DPB1. This test can be ordered to supplement historic solid organ transplant typings that are incomplete, or for disease associations for any of the Class II antigens.
Test Details
Days Set Up
Monday - Friday
Analytic Time
5 days
Soft Order Code
HLC2L
MiChart Code
HLA Class II Low Resolution (HLC2L)
Synonyms
- TTYPE
- TISSUE TYPING
- HLA Class II Low Resolution
- HLA REPORT
- HLA Antigen Typing DRB1, DRB345, DQB1, DPB1
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection
Collect specimen in sufficient yellow top (ACD) solution A tubes early in the day. Send intact specimen at room temperature. Do not refrigerate or freeze.
Alternate Specimen
Lavender top EDTA tube
Normal Volume
10 mL ACD (solution A) whole blood
Billing
CPT Code
81375
Fee Code
OA004