Real-Time Polymerase Chain Reaction (PCR)
This test is used for virologic detection of HIV-1 infection in infants <18 months of age (an age group for which serologic tests are unreliable) born to HIV-1-infected mothers, early detection of acute HIV-1 infection in children and adults who may be receiving combination antiretroviral prophylaxis or pre-emptive treatment, and determining eradication of HIV-1 in individuals receiving combination highly active antiretroviral therapies.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
This assay is optimized for the detection of HIV-1 groups M (subtypes A to H), N and O, but it may not detect all HIV-1 group N or O strains.
Diagnosis of HIV-1 infection should not rely solely upon a “Detected" result for HIV-1 DNA and/or RNA. Such a result should be considered in conjunction with patient’s clinical presentation, physical findings, and other diagnostic laboratory tests prior to establishing the diagnosis. An “Undetected" result indicates only the absence of HIV-1 DNA and RNA in the specimen tested and does not exclude the diagnosis of HIV-1 infection. “Undetected" results should be interpreted with caution, considering the patient's risk factors for HIV-1 infection, the analytical sensitivity of the assay, and the group of the infecting HIV-1 strain. Follow-up testing is recommended for high-risk patients with initially “Undetected" test results.
- HIV-1 DNA by PCR
- HIV-1 DNA by PCR, Diagnostic
- HIV-1 DNA by PCR, Qualitative
- Human Immunodeficiency Virus DNA by PCR
- HIV-1 PROVIRAL DNA BY PCR,QUAL
- HIV-1 proviral DNA
Collect specimen in a lavender top tube. Centrifuge, aliquot plasma into a plastic vial within 6 hours of collection, and refrigerate. If shipment will be delayed for >5 days, freeze plasma specimen at -70 C (up to 35 days) until shipment on dry ice.
Test sent to Mayo Medical Laboratories.