Update Type: Test Code Change
Test Updated: 08/17/2022
Test Overview
Test Methodology

Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) followed by Hybridization with Sequence-Specific, Fluorescent-Labeled Oligonucleotide Probes

Test Usage

Determining hepatitis C virus (HCV) genotype (1 to 5) to guide antiviral therapy in patients with chronic hepatitis C. Differentiating between HCV subtypes 1a and 1b.

Reference Range *

Not detected.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday
Analytic Time

1 - 6 days

Soft Order Code
MHCVG
MiChart Code
Hepatitis C Virus, Genotype
Synonyms
  • HCV Genotyping
  • Hep C genotype
  • Hepatitis C Virus Line Probe Assay
  • HVCG
  • HCVGEN
  • HEPATITIS C VIRUS, GENOTYPING
  • HEPATITIS C VIRUS, GENOTYPING
Laboratory
Sendout
Reference Laboratory
Mayo HCVG
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in an SST or red top tube. Centrifuge, aliquot serum into a polypropylene plastic vial within 2 hours of collection and freeze (preferred) or refrigerate up to 48 hours. Do not allow the specimen to thaw at any time during storage or transport, due to possible degradation of the RNA. Serum specimens previously submitted to other laboratories for non-microbiology tests are not acceptable for add-on test requests due to possible sample-to-sample carryover from automation used for those tests. Specimens should contain a recommended minimum hepatitis C virus viral load of 500 IU/mL.

Contraindications
This assay should not be used as a screening test for HCV infection. It should be performed only on specimens obtained from patients confirmed to have HCV RNA levels in serum of 500 IU/mL or higher.
Normal Volume
5 mL serum
Minimum Volume
1.5 mL serum
Additional Information

Specimens either generating indeterminate genotype results or results with multiple or mixed HCV genotypes (eg, 1, 5; 1a, 2; or 3, 5) containing genotype 1 but no subtype will be automatically evaluated with by genotype resolution at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Mayo Clinic Laboratories.

Billing
CPT Code
87902
Fee Code
AB058
Reflex CPT
87902
Reflex Fee Code
AB059
LOINC
32286-7