Test Overview
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) followed by Hybridization with Sequence-Specific, Fluorescent-Labeled Oligonucleotide Probes
Determining hepatitis C virus (HCV) genotype (1 to 5) to guide antiviral therapy in patients with chronic hepatitis C. Differentiating between HCV subtypes 1a and 1b.
Not detected.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Test Details
1 - 6 days
- HCV Genotyping
- Hep C genotype
- Hepatitis C Virus Line Probe Assay
Specimen Requirements
Collect specimen in an SST or red top tube. Centrifuge, aliquot serum into a polypropylene plastic vial within 2 hours of collection and freeze (preferred) or refrigerate up to 48 hours. Do not allow the specimen to thaw at any time during storage or transport, due to possible degradation of the RNA. Serum specimens previously submitted to other laboratories for non-microbiology tests are not acceptable for add-on test requests due to possible sample-to-sample carryover from automation used for those tests. Specimens should contain a recommended minimum hepatitis C virus viral load of 500 IU/mL.
Additional Information
Specimens either generating indeterminate genotype results or results with multiple or mixed HCV genotypes (eg, 1, 5; 1a, 2; or 3, 5) containing genotype 1 but no subtype will be automatically evaluated with by genotype resolution at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Mayo Clinic Laboratories.