Test Overview
Test Methodology

Heparin-associated antibodies to PF4 detected by an ELISA technique.

Test Usage

Detection of heparin-dependent platelet antibodies.

Reference Range *

0.000 - 0.399. Negative

Test Details
Days Set Up
Daily, one run per day with the exception of holidays.
The test is not run on holidays, but will be run the next business day instead.
Analytic Time

3 hours

Soft Order Code
HITAB
MiChart Code
Heparin-Induced Platelet Antibody
Synonyms
  • Platelet Factor 4 Antibody
  • PF4 Antibody
  • Heparin Antibodies
  • Heparin Associated Throbocytopenia and Thrombosis
  • Heparin Associated Thrombosis
  • Heparin Associated Thrombosis (HAT)
  • Heparin Dependent Antiplatelet Antibodies
  • Heparin Dependent Anti-Platelet Antibodies
  • Heparin Induced Thrombosis (HIT)
  • Heparin-Associated Thrombocytopenia and Thrombosis
  • Platelet Antibody, Heparin Dependent
  • HIT ELISA
  • HEPARIN ANTIBODY ASSAY
  • Heparin Associated Platelet Antibody
Laboratory
Hematopathology
Section
Coagulation
STAT Availability

STAT testing is not available for this test. The decision to discontinue heparin therapy is a clinical one and should not be delayed due to a pending HIT antibody test or serotonin-release assay. Please see the clinical HIT guideline for details. https://pharmwebsp.med.umich.edu/_layouts/15/WopiFrame.aspx?sourcedoc=/…

Specimen Requirements
Collection Instructions

Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 3 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic tube, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

Blue Top Tube
Rejection Criteria
Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, or contaminated with heparin.
Normal Volume
Full 2.7 mL tube or 1 mL plasma aliquot
Minimum Volume
Full 1.8 mL tube or 1 mL plasma aliquot
Storage Temperature
Room temperature for whole blood or frozen aliquot of citrated plasma.
Additional Information

The heparin antibody assay is intended as a screening test and should not be relied upon solely to establish the diagnosis of heparin-induced thrombocytopenia.The decision to discontinue heparin therapy is a clinical one and should not be delayed due to a pending HIT antibody test or serotonin-release assay. Please see the clinical HIT guideline for details. https://pharmwebsp.med.umich.edu/_layouts/15/WopiFrame.aspx?sourcedoc=/…

Billing
CPT Code
86022
Fee Code
31142
LOINC
6002-0
NY State Approved
No