Test Overview
Test Methodology

Molecular Typing (NGS).

Test Usage

Hematopoietic Cell Transplant (HCT) recipients are typed for HLA-A, -B, -C, -DRB1, -DRB3/4/5, -DQB1, -DQA1, -DPB1, -DPA1, loci by high resolution (allele level) molecular methods.

In addition to the HLA high resolution typing patients are screened for preformed anti-HLA antibodies, the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection.

Test Details
Days Set Up
Monday - Friday, 7:00am - 4:00pm
Analytic Time

5 days

Soft Order Code
HLHR, HLPRA
Synonyms
  • Bone Marrow Transplant Recipient
  • HLA Class I High Resolution
  • HLA High Resolution Typing
  • HLA Antibody Screen Mixed
  • HLA Screening Class I & II
  • HLA Class II High Resolution
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send specimens at room temperature. Do not refrigerate or freeze.

Normal Volume

HLHR: 2 full tubes, Yellow top (ACD) solution A tube
HLPRA: 1 full tube, Red Top/SST
or 4 buccal swabs

Minimum Volume

HLHR: 2 full tubes, Yellow top (ACD) solution A tube
HLPRA: 1 full tube, Red Top/SST
or 4 buccal swabs

Storage Temperature
Room Temperature
Rejection Criteria
Frozen samples, mislabeled samples, samples that do not have the required two patient identifiers.
Billing
CPT Code
81379, 81382 X5 , 86830, 86831
Fee Code
OA008, OA009 x5, OA010, OA011
Reflex CPT
86832, 86833
Reflex Fee Code
37962, 37963
NY State Approved
No