Test Overview
Test Methodology

Sequence Specific Oligonucleotide Polymorphism (SSOP) and Next Generation Sequencing (NGS).

Test Usage

Hematopoietic Cell Transplant (HCT) recipients are typed for HLA-A, -B, -C, -DRB1, -DRB3/4/5, -DQB1, -DQA1, -DPB1, -DPA1, loci by high resolution (allele level) molecular methods.

In addition to the HLA high resolution typing patients are screened for preformed anti-HLA antibodies, the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
HLHR, HLAAB
Synonyms
  • HCTNP
  • Bone Marrow Transplant Recipient
  • New Patient, HC Tx
  • HLA Class I High Resolution
  • HLA High Resolution Typing
  • HLA Antibody Screen Mixed
  • HLHR
  • HLASM
  • HLA Screening Class I & II
  • HLA REPORT
  • HLA Class II High Resolution
  • TTYPE
  • TISSUE TYPING
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze.

Alternate Specimen
Lavender top tubes (EDTA), buccal swabs
Normal Volume
HLLR: 2 x 10 mL Yellow top (ACD) solution A tube
HLAAB: 10 mL Red Top or SST tube
Minimum Volume
HLLR: 5 mL Yellow top (ACD) solution A tube
HLAAB: 3 mL Red top or SST tube
Storage Temperature
Room Temperature
Rejection Criteria
Specimens received in the incorrect tube for tests requested will be rejected.
Specimens that are not properly labeled with 2 identifiers will be rejected.
Insufficient sample amounts will be rejected.
Additional Information

Test includes HLA Typing Class I & II and Antibody Screening Class I & II. HLA Antibody Specificity Class I and II will be performed if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

HLA Typing should only be ordered once every five years.

Billing
CPT Code
81379, 81382 X5 , 86830, 86831
Fee Code
OA008, OA009 x5, OA010, OA011
Reflex CPT
86832, 86833
Reflex Fee Code
37962, 37963