Test Overview
Test Methodology

Sequence Specific Oligonucleotide Polymorphism (SSOP) and Next Generation Sequencing (NGS).

Test Usage

Hematopoietic Cell Transplant (HCT) recipients are typed for HLA-A, -B, -C, -DRB1, -DRB3/4/5, -DQB1, -DQA1, -DPB1, -DPA1, loci by high resolution (allele level) molecular methods.

In addition to the HLA high resolution typing patients are screened for preformed anti-HLA antibodies, the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

3 - 10 days

Soft Order Code
  • Bone Marrow Transplant Recipient
  • New Patient, HC Tx
  • HLA Class I High Resolution
  • HLA High Resolution Typing
  • HLA Antibody Screen Mixed
  • HLHR
  • HLA Screening Class I & II
  • HLA Class II High Resolution
Specimen Requirements

Collect specimens in sufficient yellow top (ACD) solution A tube AND SST tubes. Send intact specimens at room temperature. Do not refrigerate or freeze.

Alternate Specimen
Yellow top ACD Solution B, Lavender top tubes (EDTA), buccal swabs
Normal Volume
HLLR: 20 mL Yellow top (ACD) solution A tube
PRAMO: 5 mL SST tube
Minimum Volume
HLLR: 5 mL Yellow top (ACD) solution A tube
PRAMO: 3 mL SST tube
Storage Temperature
Stored at -20° to -80° C
Rejection Criteria
Specimens received in the incorrect tube for tests requested will be rejected.
Specimens that are not properly labeled with 2 identifiers will be rejected.
Insufficient sample amounts will be rejected.
Additional Information

Test includes HLA Typing Class I & II and Antibody Screening Class I & II. HLA Antibody Specificity Class I and II will be performed if indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

HLA Typing should only be ordered once every five years.

CPT Code
81379, 81382 X5 , 86830, 86831
Fee Code
OA008, OA009 x5, OA010, OA011
Reflex CPT
86832, 86833
Reflex Fee Code
37962, 37963