Test Overview
Test Methodology

Molecular Typing (SSOP, and SSP/SBT if needed)

Test Usage

At the UMHS Histocompatibility Laboratory potential recipients are typed for HLA-A, B, C, DRB1,3,4,5, DQB1, loci by high resolution (allele level) molecular methods. The methods utilized for HLA typing are SSOP, SSP and/or SBT. In addition to the HLA high resolution typing patients are screened for preformed anti-HLA antibodies, the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection. The HCT donor could be family member known as related donor or an unrelated donor. RELATED DONOR: Half of the HLA alleles or haplotype are inherited from the mother and half from the father, so each recipient’s sibling who has the same parents has a 25% chance of matching. There is less chances to find a matched donor in the extended family members, however, in cases where the recipient carry a rare allele or haplotype in combination with a frequent haplotype is recommended extend the searching to relatives such as grandparents, aunts, uncles or cousins. Related donor donors are initially screened by low resolution techniques for HLA-A,B and DRB1. For those 6/6 or 5/6 matched donors a high resolution confirmatory typing for HLA-A, B, C, DRB1,3,4,5, DQB1will be performed. High-resolution typing results are reported to transplant physicians for final selection. UNRELATED DONOR: About 70% of patients who need a transplant do not have a suitable related donor. For these patients the high resolution typing for HLA-A, B, C, DRB1,3,4,5, DQB1 a searching request is submitted to the National Marrow Donor Program (NMDP) and other registries around the world, to find a matched unrelated donor among the nearly 18.5 million voluntary donors and more than 590,000 cord blood units. When an unrelated matched donor is identified a sample is shipped to our laboratory for a confirmatory high resolution typing. Possible donors high-resolution typing results are reported to transplant physicians for final selection.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

3 - 10 days

Soft Order Code
HLBML
MiChart Code
HLA Donor Low Res Typing
Synonyms
  • Bone Marrow Transplant Donor
  • New Donor, HC Tx
  • HCTND
  • HLA Class I Low Resolution
  • HLA Class II Low Resolution
  • HLA REPORT
  • HLBML
  • TTYPE
  • TISSUE TYPING
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in sufficient yellow top (ACD) solution A tubes. Send intact specimen at room temperature. Do not refrigerate or freeze.

Alternate Specimen
Yellow top ACD Solution B, Green top tube (sodium or lithium heparin), Lavender top tubes (EDTA).
Normal Volume
10 mL ACD (solution A) or EDTA whole blood or 4 buccal swabs. Patients with extremely low white counts may require additional specimen.
Minimum Volume
5 mL ACD (solution A) or EDTA whole blood or 2 buccal swabs. Patients with extremely low white counts may require additional specimen.
Additional Information

By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Hematopoietic cell transplants (HCT) is a potentially curative therapy for cancers of the blood and bone marrow, including leukemias, lymphomas, and multiple myeloma. Use of HCT has increased as new techniques have allowed for transplantation in patients who previously would not have been considered HCT candidates. Among the most important factors that affect the outcome of the HCT is the degree of human leukocyte antigen (HLA) matching between the recipient and the related or unrelated donor, or cord blood unit. HLA matching plays an important role in engraftment, occurrence and severity of graft-versus-host disease (GVHD) and overall survival.

Billing
CPT Code
81372, 81376
Fee Code
37921, 37922
Reflex CPT
81379, 81382
Reflex Fee Code
37942, 37959
Resources