Test Overview
Test Methodology

Sequence Specific Oligonucleotide Polymorphism (SSOP) and Next Generation Sequencing (NGS).

Test Usage

Hematopoietic Cell Transplant (HCT) recipients and respective donors are typed for HLA-A, -B, -DRB1 by low resolution (antigen level) molecular methods. If matched with recipient, donors are typed by high resolution (allele level) molecular to include the following loci: HLA-A, -B, -DRB1, -DRB3/4/5, -DQB1, -DQA1, -DPB1, -DPA1.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

3 - 10 days

Soft Order Code
HLBML
MiChart Code
LAB7869 HLA Donor Low Res Typing
Synonyms
  • Bone Marrow Transplant Donor
  • New Donor, HC Tx
  • HCTND
  • HLA Class I Low Resolution
  • HLA Class II Low Resolution
  • HLA REPORT
  • HLBML
  • TTYPE
  • TISSUE TYPING
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in sufficient yellow top (ACD) solution A tubes. Send intact specimen at room temperature. Do not refrigerate or freeze.

Alternate Specimen
None
Normal Volume
10 mL ACD (solution A) or EDTA whole blood or 4 buccal swabs. Patients with extremely low white counts may require additional specimen.
Minimum Volume
5 mL ACD (solution A) or EDTA whole blood or 2 buccal swabs. Patients with extremely low white counts may require additional specimen.
Storage Temperature
Stored at -20° to -80° C
Rejection Criteria
Specimens received in the incorrect tube for tests requested will be rejected.
Specimens that are not properly labeled with 2 identifiers will be rejected.
Insufficient sample amounts will be rejected.
Additional Information

By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Hematopoietic cell transplants (HCT) is a potentially curative therapy for cancers of the blood and bone marrow, including leukemias, lymphomas, and multiple myeloma. Use of HCT has increased as new techniques have allowed for transplantation in patients who previously would not have been considered HCT candidates. Among the most important factors that affect the outcome of the HCT is the degree of human leukocyte antigen (HLA) matching between the recipient and the related or unrelated donor, or cord blood unit. HLA matching plays an important role in engraftment, occurrence and severity of graft-versus-host disease (GVHD) and overall survival.

Billing
CPT Code
81372, 81376
Fee Code
37921, 37922
Reflex CPT
81371
Reflex Fee Code
37591