Test Overview
Test Methodology

Solid Phase Assays

Test Usage

In addition to HLA high resolution typing, patients are screened for preformed anti-HLA antibodies; the detection of anti-HLA antibodies directed to possible mismatches in less ideal donors is related with delay in the platelets and neutrophil engraftment and even with graft rejection. Panel reactivity antibody (PRA) indicates the degree of reactivity of tested serum in terms of percent of positive reactions when tested against a panel of known HLA phenotypes.

Reference Range *

For positive results, the report will define the detected HLA antibody specificities. For negative results, 0% PRA will be reported.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:30pm
Analytic Time

5 days

Soft Order Code
HLASM
Synonyms
  • Allogenic PRA
  • PRAAL
  • TTYPE
  • Allogenic Bone Marrow Transplant
  • HLASM
  • HLA Antibody Screen Mixed
  • TISSUE TYPING
  • HLA REPORT
  • HLA Antibody Screening Class I & II
Laboratory
Histocompatibility/Immunogenetics
Section
Histocompatibility
Specimen Requirements
Collection

Collect specimen in a red top (preferred) or SST tube. Send intact specimen at room temperature. Do not refrigerate or freeze.

Yellow Top Tube
Normal Volume
5 mL serum
Minimum Volume
3 mL serum
Additional Information

The Class I & II screening assay is performed first and detects the presence/absence of HLA antibodies. If the screening assay is positive, a high specificity and sensitivity single HLA Antigen assay will be performed to identify the specificity of the anti-HLA antibody present in the serum sample from the recipient. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

Billing
CPT Code
86828
Fee Code
37943
Reflex CPT
86832, 86833
Reflex Fee Code
37962, 37963
NY State Approved
No