Kinetic Spectrophotometry (SP)
Evaluation of individuals with episodic or chronic Coombs-negative nonspherocytic hemolytic anemia
Rapid testing to assess glucose 6-phosphate dehydrogenase (G6PD) enzyme capacity prior to Rasburicase or other therapies that may cause hemolysis or methemoglobinemia in G6PD deficient patients
May aid in the creation of a comprehensive patient profile and can ensure appropriate patient monitoring for developing anemia
> or =12 months of age: 8.0-11.9 U/g Hb. Reference values have not been established for patients who are less than 12 months of age.
During hemolytic events normal glucose 6-phosphate dehydrogenase (G6PD) activity values may be measured for several weeks following hemolysis.
Reticulocytosis from any cause can mask some G6PD deficiency cases by raising the activity level. Comparison to other red blood cell enzyme activity levels may be useful.
Recent transfusion may mask the patient’s intrinsic enzyme activity and cause unreliable results.
- Glucose-6-Phosphate Dehydrogenase Deficiency
- G6PD, Quantitative
- Glucose-6-Phosphate Dehydrogenase, Quantitative
Collect specimen in a yellow top (ACD) solution B tube preferred; Lav top (EDTA) or ACD solution A are acceptable. Send 6.0 mL of intact whole blood. Refrigerate. Do not transfer blood to other containers.
Test sent to Mayo Clinic Laboratories.