Test Overview
Test Methodology

Kinetic Spectrophotometry (SP)

Test Usage

Evaluation of individuals with episodic or chronic Coombs-negative nonspherocytic hemolytic anemia
Rapid testing to assess glucose 6-phosphate dehydrogenase (G6PD) enzyme capacity prior to Rasburicase or other therapies that may cause hemolysis or methemoglobinemia in G6PD deficient patients
May aid in the creation of a comprehensive patient profile and can ensure appropriate patient monitoring for developing anemia

Reference Range *

> or =12 months of age: 8.0-11.9 U/g Hb. Reference values have not been established for patients who are less than 12 months of age.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

During hemolytic events normal glucose 6-phosphate dehydrogenase (G6PD) activity values may be measured for several weeks following hemolysis.
Reticulocytosis from any cause can mask some G6PD deficiency cases by raising the activity level. Comparison to other red blood cell enzyme activity levels may be useful.
Recent transfusion may mask the patient’s intrinsic enzyme activity and cause unreliable results.

Test Details
Soft Order Code
G6PD1
MiChart Code
G-6-PD, Erythrocytes
Synonyms
  • Glucose-6-Phosphate Dehydrogenase Deficiency
  • G6PD, Quantitative
  • Glucose-6-Phosphate Dehydrogenase, Quantitative
  • G6PDQ
Laboratory
Sendout
Reference Laboratory
Mayo G6PD1
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a yellow top (ACD) solution B tube preferred; Lav top (EDTA) or ACD solution A are acceptable. Send 6.0 mL of intact whole blood. Refrigerate. Do not transfer blood to other containers.

Alternate Specimen
Lavender top (EDTA) and ACD- Solution A are also acceptable.
Normal Volume
6 mL ACD (solution B) whole blood preferred; Lav top (EDTA) or ACD solution A are acceptable
Minimum Volume
1 mL ACD (solution B) whole blood preferred; Lav top (EDTA) or ACD solution A are acceptable
Additional Information

Test sent to Mayo Clinic Laboratories.

Billing
CPT Code
82955
Fee Code
22033
LOINC
32546-4