The assay is based upon a modification of the Limulus Amebocyte Lysate (LAL) pathway. The key assay reagent is modified to eliminate Factor C, and is therefore specific for (1,3)-beta-D-glucan.
The Fungitell beta-D Glucan assay is indicated for the presumptive diagnosis of invasive fungal disease through detection of elevated levels of (1,3)-beta-D glucan in serum. The Fungitell assay detects (1,3)-beta-Dglucan from the following pathogens: Candida spp., Acremonium, Aspergillus spp., Coccidioides immitis, Fusarium spp., Histoplasma capsulatum, Trichosporon spp., Sporothrix schenckii, Saccharomyces cerevisiae, and Pneumocystis jiroveci.
Negative: Less than 60 pg/mL. Indeterminate: 60 - 79 pg/mL. Positive: Greater than or equal to 80 pg/mL.
The Fungitell assay does not detect certain fungal species such as the genus Cryptococcus, which produces very low levels of (1,3)-beta-D glucan, nor the Zygomycetes, such as Absidia, Mucor, and Rhizopus, which are not known to produce (1,3)-beta-D glucan. Studies indicate Blastomyces dermatitidis is usually not detected due to little (1,3)-beta-D glucan produced in the yeast phase.
2 - 4 days
- 1,3 beta D glucan
- Fungitell beta-D Glucan assay
Collect specimen in an SST tube. Centrifuge specimen within 2 hours of collection to pellet cells below the gel and freeze unopened tube.
Freeze unopened tube after centrifugation. Do not aliquot.
If the test result is greater than 500 pg/mL, a titer (VRFNG) will be performed at an additional charge. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated. Test sent to Viracor Eurofins.