Real-Time Polymerase Chain Reaction (PCR)/TaqMan DNA Probe Hybridization
Aids in the diagnosis of primary amebic meningoencephalitis and granulomatous
amebic encephalitis in spinal fluid and tissue in conjunction with clinical findings.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Primary amebic meningoencephalitis due to Naegleria fowleri is a rapidly fatal disease, and prompt detection may improve the likelihood of survival. The fastest way to make a diagnosis is through examination of spinal fluid for characteristic trophozoites. This may be performed locally by obtaining a cell count with manual differential (hematology laboratory); alert the laboratory that amebic infection is suspected.
- CSF, Fresh Water Amoeba Exam
- Fresh Water Amoeba Culture
- Balamuthia mandrillaris PCR
- Naegleria fowleri PCR
- Acanthamoeba species PCR
Submit only one of the following specimens: 1) CSF in a sterile container (vial #2), 2) brain, skin, or lung tissue in a sterile container with 1 mL of sterile saline or minimal essential media (MEM), 3) formalin-fixed paraffin-embedded (FFPE) brain, skin, or lung tissue in a sterile container. Refrigerate.
Test includes PCR for Acanthamoeba species, Naegleria fowleri, and Balamuthia mandrillaris. Test sent to Mayo Medical Laboratories.