Real-Time Polymerase Chain Reaction (PCR)/TaqMan DNA Probe Hybridization
Aids in the diagnosis of primary amebic meningoencephalitis and granulomatous
amebic encephalitis in spinal fluid and tissue in conjunction with clinical findings.
Primary amebic meningoencephalitis due to Naegleria fowleri is a rapidly fatal disease, and prompt detection may improve the likelihood of survival. The fastest way to make a diagnosis is through examination of spinal fluid for characteristic trophozoites. This may be performed locally by obtaining a cell count with manual differential (hematology laboratory); alert the laboratory that amebic infection is suspected.
- CSF, Fresh Water Amoeba Exam
- Fresh Water Amoeba Culture
- Balamuthia mandrillaris PCR
- Naegleria fowleri PCR
- Acanthamoeba species PCR
Submit only one of the following specimens: 1) CSF in a sterile container (vial #2), 2) brain, skin, or lung tissue in a sterile container with 1 mL of sterile saline or minimal essential media (MEM), 3) formalin-fixed paraffin-embedded (FFPE) brain, skin, or lung tissue in a sterile container. Refrigerate.
Test includes PCR for Acanthamoeba species, Naegleria fowleri, and Balamuthia mandrillaris. Test sent to Mayo Medical Laboratories.