Test Overview
Test Methodology

Flow Cytometry

Test Usage

Mutations of the FoxP3 gene have been described in patients with immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome (IPEX). This assay uses flow cytometry to determine the proportion and intensity of intracellular FoxP3 protein expression in the subset of CD4+ T cells that are CD25 bright and CD127 negative. The PCH101 antibody reacts with the amino terminus of human foxp3 protein.

Test Limitations

This flow cytometry assay is intended to be used as a screening test. Screening tests are neither 100 percent sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient’s clinical presentation suggests that the probability of a diagnosis is high.

Test Details
Days Set Up
Monday - Friday
Analytic Time

5 - 7 days

Soft Order Code
Reference Laboratory
Cincinnati Children’s Hospital 2904100
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in lavendar top tube and send intact whold blood specimen at room temperature. Include a copy of the patient's concurrently drawn CBC and Differential result. Draw Monday-Thursday only. Please call a STAT courier to bring it to Specimen Processing

Special Handling

Draw Monday-Thursday only. The label states between 12-4pm for the collection time. That is so it can be delivered to Specimen Processing and still get shipped out that day. Please ask sendouts before canceling the specimen if it can still be shipped. If collecting offsite, please call a STAT courier to bring it to Specimen Processing

Normal Volume
3 mL EDTA whole blood
Minimum Volume
1 mL EDTA whole blood
Additional Information

Test sent to Cincinnati Children’s Hospital.

CPT Code
88184, 88185 x3
Fee Code
AA709, AA710 x3