2 - 7 days
FLT3 Gene Mutation Analysis
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Multiplex Polymerase Chain Reaction (PCR) with Capillary Electrophoresis
The FLT3 gene encodes a cell surface receptor tyrosine kinase that is expressed on early hematopoietic stem cells. Activating mutations in FLT3 occur in approximately 25-30% of patients with acute myelogenous leukemia (AML). The mutation generally occurs as either an internal tandem duplication (ITD) within the juxtamembrane domain, or as a missense point mutation within the tyrosine kinase domain (TKD) at codon 835. This test detects both the FLT3-ITD and FLT3-TKD (codon 835) mutations. The presence of a FLT3-ITD mutation has been associated with a significantly poorer outcome in normal karyotype AML.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Collect blood or bone marrow in a lavender top tube. Refrigerate and send intact blood or bone marrow specimen within 48 hours of collection. Fresh tissue (preferably 0.5cm3, sent in RPMI) and fresh aspirates or body fluids are acceptable. Refrigerate and send, preferably within 24 hours. Frozen tissue specimens – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fresh cell suspensions in RPMI should be refrigerated and sent, preferably within 48 hours. Frozen cell suspensions – preferably frozen with 1 hour of collection – may also be sent frozen on dry ice. Fixed cytogenetic cell suspensions or pellets in Carnoy’s fixative. Refrigerate and send.