Days Set Up
Monday - Friday, 8:30 am - 5:30 pm
Analytic Time

1 week

MiChart Code
Mulitprobe FISH for Melanoma (Dermatopathology Molecular Diagnostics)
Soft Order Code

Test Updated:


FISH panel 6p25 (RREB1), 6q23 (MYB), 11q13 (CCND1) and CEP6
FISH panel 9q21 (CDKN2A) and CEP9
FISH for 8q24 (MYC)
Multiplex FISH for Melanoma
BAP-1 / BAP1 IHC Stain

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Test Overview

Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test is designed to detect copy number abnormalities at probed loci via fluorescence in situ hybridization (FISH) in FFPE tissue specimens. The test is used to aid in the diagnosis of histologically ambiguous melanocytic lesions; a positive result favors a diagnosis of melanoma while a negative result one of nevus. Published data report a sensitivity of 86-100% and a specificity of 76-98% for the diagnosis of melanoma in selected cohorts including atypical spitzoid neoplasms. Three multiprobe FISH panels are available. Panel 1 include probes 6p25 (RREB1), 6q23 (MYB), 11q13 (CCND1) and CEP6; panel 2 include 9q21 (CDKN2A) and CEP9; panel 3 include 8q24 (MYC). Panel 1 is recommended for all histologically ambiguous melanocytic tumors. The addition of panels 2 and 3 increases assay sensitivity for selected melanocytic neoplasms including spitzoid tumors and nevoid melanomas. A positive result is defined as percent of nuclei with abnormal signal counts exceeding cut-off criteria for any of the probes. Results from this test should not be used alone in diagnosing a melanocytic lesion and correlation with clinical history, histological examination and other standard diagnostic procedures is necessary for diagnosis.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Submit a formalin-fixed, paraffin-embedded (FFPE) block containing tumor tissue (preferred specimen). If a block is not available submit 3 FFPE unstained sections at 4-micron thickness placed on electrostatic-coated slides, unbaked and one regular H&E obtained as serial sections; store at room temperature.

Minimum Volume

Billing Information

CPT Code
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.