Test Overview

Test Methodology

Multiplex Flow Immunoassay

Test Usage

Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 10 possible detectable antibody specificities. MLabs promotes a more cost effective strategy an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). These antibodies will account for the vast majority of significant positive results. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates. The laboratory recommends either anti-dsDNA (order code ADNA) or crithidia DNAIFA (order code DNAIF) to detect and quantify anti-dsDNA.

Reference Range*

Negative

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed

Monday - Friday

Analytic Time

1 day

Soft Order Code

ENA5/ENA7/ENA10

Synonyms

Synonyms

SmRNP Antibody
RIBOP
ENAB
Sjogren's Antibodies
U1RNP Antibody
Centromere B Antibody
Anti-Centromere
Scl-70 Antibody
SCL-70
ANTI SCLERODERMA AB TO 70 AG
JO-1
AUTOANTIBODIES TO JO1 ANTIGEN
ANTI-RNP
RNP
ANTI-SmRNP
SMRNP
ANTI-SM
CENT B
SS-B
ANTI-SSA/ANTI-RO
ENA (ANTI SS-A/ANTI SS-B)
SMRN
ENA (ANTI-Sm/SmRNP/RNP)
ENA5
ENA (Anti-SM/RNP/SS-A/SS-B)
ENA7
ENA 7 ANTIBODY PANEL
SMITH
CENTROMERE B
ANTI-SSB/ANTI-LA
SS-A
ENA11
ENA 11 ANTIBODY PANEL
CHROM
CHROMATIN
RIBO P
RIBOSOMAL PROTEIN
SSASSB
Anti-Scl 70
Sjogrens syndrome-A extractable nuclear Ab
Autoantibodies to Jo-1 Antigen
Jo-1 Antibody
Chromatin Antibody
Ribosomal Protein Antibody
Anti-SSB (La)
Anti-SSA (Ro)
Ro Antibody
La Antibody
RNP Antibody
Anti-Ro (SSA)
Anti-RNP (Ribonucleoprotein)
Anti-Sm (Smith)
Sm (Smith) Antibody
Anti-La (SSB)
SS-A Antibody
SS-B Antibody
Anti-SmRNP
Anti-Jo 1
Scleroderma Antibody
EXTRACTABLE NUCLEAR ANTIBODIES
Anti-Synthetase Syndrome
Anti-HisRS (Jo-1)
Anti-tRNA Synthetase
Sjogrens syndrome-B extractable nuclear Ab
Ribonucleoprotein extractable nuclear Ab
Smith extractable nuclear Ab
SCL70
JO1
CENTB
RIBOP
SSA
SSB
SSAB
ENA10

+ See More

Laboratory

Chemical Pathology

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Specimen Requirements

Offsite Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.

Container

Yellow Top Tube

Normal Volume

0.5 mL serum

Minimum Volume

0.25 mL serum

Additional Information

Due to the known incidence of false positive SCL-70 results by the multiplex immunoassay method in patients without characteristic scleroderma symptoms, specimens positive for SCL-70 will be sent to RDL Reference Laboratories for more specific SCL-70 ELISA testing at an additional charge (order code RSCL). If the EIA test is positive, immunodiffusion testing will be performed for confirmation at an additional charge (order code RSCLR). A separate report will be issued for the confirmatory test. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.
See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA10 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENTB), Jo-1 (JO1), Ribosomal P (RIBOP), RNP (RNP), Scleroderma (SCL70), Smith (SMITH), SS-A (SSA), and SS-B (SSB).

Billing Information

Fee Codes


CPT Code

86235 EACH ANTIBODY

Reflex Fee Code

AA289 RSCL, AA371 RSCRL

LOINC

Z32-3 ENA5, Z1002-5 ENA7, 8076-2 JO-1, 8092-9 SCL-70, 16137-2 CENT B, Z1001-7 CHROM, 5130-0 DSDNA, 31592-9 RIBO P

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