Test Overview
Multiplex Flow Immunoassay
Useful diagnostic and prognostic information can be gained in ANA positive patients by knowing the specificity of the autoantibodies that are detected. Ideally, collagen vascular disease diagnosis is based on a combination of clinical symptoms and laboratory testing. It is not cost effective to follow up every positive ANA with assays for all 10 possible detectable antibody specificities. MLabs promotes a more cost effective strategy an ENA 5 Antibody Panel (includes SS-A, SS-B, Sm, RNP, and SmRNP) (order code ENA5). These antibodies will account for the vast majority of significant positive results. Other ENA antigens such as Scl-70, Centromere B, Jo-1, and Ribosomal P should not be routinely screened for, but ordered as individual tests as the differential diagnosis dictates. The laboratory recommends either anti-dsDNA (order code ADNA) or crithidia DNAIFA (order code DNAIF) to detect and quantify anti-dsDNA.
Negative
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Test Details
1 day
- SmRNP Antibody
- RIBOP
- ENAB
- Sjogren's Antibodies
- U1RNP Antibody
- Centromere B Antibody
- Anti-Centromere
- Scl-70 Antibody
- SCL-70
- ANTI SCLERODERMA AB TO 70 AG
- JO-1
- AUTOANTIBODIES TO JO1 ANTIGEN
- ANTI-RNP
- RNP
- ANTI-SmRNP
- SMRNP
- ANTI-SM
- CENT B
- SS-B
- ANTI-SSA/ANTI-RO
- ENA (ANTI SS-A/ANTI SS-B)
- SMRN
- ENA (ANTI-Sm/SmRNP/RNP)
- ENA5
- ENA (Anti-SM/RNP/SS-A/SS-B)
- ENA7
- ENA 7 ANTIBODY PANEL
- SMITH
- CENTROMERE B
- ANTI-SSB/ANTI-LA
- SS-A
- ENA11
- ENA 11 ANTIBODY PANEL
- CHROM
- CHROMATIN
- RIBO P
- RIBOSOMAL PROTEIN
- SSASSB
- Anti-Scl 70
- Sjogrens syndrome-A extractable nuclear Ab
- Autoantibodies to Jo-1 Antigen
- Jo-1 Antibody
- Chromatin Antibody
- Ribosomal Protein Antibody
- Anti-SSB (La)
- Anti-SSA (Ro)
- Ro Antibody
- La Antibody
- RNP Antibody
- Anti-Ro (SSA)
- Anti-RNP (Ribonucleoprotein)
- Anti-Sm (Smith)
- Sm (Smith) Antibody
- Anti-La (SSB)
- SS-A Antibody
- SS-B Antibody
- Anti-SmRNP
- Anti-Jo 1
- Scleroderma Antibody
- EXTRACTABLE NUCLEAR ANTIBODIES
- Anti-Synthetase Syndrome
- Anti-HisRS (Jo-1)
- Anti-tRNA Synthetase
- Sjogrens syndrome-B extractable nuclear Ab
- Ribonucleoprotein extractable nuclear Ab
- Smith extractable nuclear Ab
- SCL70
- JO1
- CENTB
- RIBOP
- SSA
- SSB
- SSAB
- ENA10
Specimen Requirements
Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Please indicate panel or specific antibody testing to be performed.
Additional Information
Due to the known incidence of false positive SCL-70 results by the multiplex immunoassay method in patients without characteristic scleroderma symptoms, specimens positive for SCL-70 will be sent to RDL Reference Laboratories for more specific SCL-70 ELISA testing at an additional charge (order code RSCL). If the EIA test is positive, immunodiffusion testing will be performed for confirmation at an additional charge (order code RSCLR). A separate report will be issued for the confirmatory test. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.
See Extractable Nuclear Antibody Testing.available online at http://www.pathology.med.umich.edu/handbook/Tables/ANA-ENA-08.pdf. ENA5 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, and RNP. ENA7 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, and Scleroderma (Scl-70). ENA10 Panel includes SS-A (Ro), SS-B (La), Smith, SmRNP, RNP, Jo-1, Scleroderma (Scl-70), Centromere B, Chromatin, and Ribosomal P. The following antibodies may also be ordered individually: Centromere B (CENTB), Jo-1 (JO1), Ribosomal P (RIBOP), RNP (RNP), Scleroderma (SCL70), Smith (SMITH), SS-A (SSA), and SS-B (SSB).