Radioimmunoassay (RIA) following hydrolysis and extraction.
Males: 3.0 - 8.0 mcg/24 hours; Females: 4.0 - 30.0 mcg/24 hours.
7 - 10 days
Collect 24 hour urine specimen with no preservative. Aliquot 10 mL into a plastic urine container and freeze. Record total 24 hour urine volume and collection dates/times on request form. Patient should not be on any steroid, ACTH, gonadotropin or Estrogen medications, if possible, for at least 48 hours prior to collection of specimen. Oral contraceptives and estrogen replacement therapy may influence Estrone levels.
Estrone is excreted into the urine in several different conjugated forms and also as unconjugated Estrone. This assay measures the total of the conjugated and unconjugated forms of Estrone. Aging and obesity are two major factors controlling production of Estrone. In postmenopausal women, very little glandular Estrone is produced. The levels found usually come from peripheral conversion of Androstenedione. Estrone exhibits a biphasic secretion with levels higher in the luteal phase. Urine Estrone measurements give an integrated picture of Estrone production and activity over a 24 hour interval. Test sent to Mayo Medical Laboratories; performed by InterScience Institute.