Test Overview
Test Methodology


Test Usage

Estrogens are important in the development and maintenance of the female sex organs and secondary sex characteristics. They also regulate the menstrual cycle and are important in the maintenance of pregnancy. Estradiol is the most potent naturally occurring estrogen. Estradiol is normally metabolized into estrone, which in turn can be metabolized along two alternative pathways eventually to either estriol or 2-methoxyestrone. The direction of estradiol metabolism is altered in hyperthyroidism and anorexia nervosa in increased formation of 2-hydroxyestrone and subsequently 2-methoxyestrone. This causes a consequent decrease in the formation of estriol.

Reference Range *

Age and sex specific reference ranges provided with report. Includes Estrone (E1) and Estradiol (E2).

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Saturday
Analytic Time

2 - 4 days

Soft Order Code
MiChart Code
Estrogens, Fractionated
  • ESTF
  • Estrogens, E1+E2, Fractionated
Reference Laboratory
Mayo ESTF (84230)
Special Testing
Specimen Requirements

Collect specimen in red top tube; do not use SST tube. Centrifuge and aliquot serum into a plastic vial within 2 hours of collection. Refrigerate.

Normal Volume
1.2 mL serum
Minimum Volume
0.8 mL serum
Rejection Criteria
SST tube not acceptable.
Additional Information

Test sent to Mayo Medical Laboratories.

CPT Code
82670, 82679
Fee Code