Test Overview
Test Methodology

Chemiluminescent Immunoassay (CLIA)

Test Usage

Assessment of hypothalmic and pituitary function; to assess ovarian failure and follicular maturation. Amenorrhea work-up: low result, if gonadotropins high, may indicate ovarian failure, if gonadotropins and estradiol low, consider hypothalamic or pituitary disorder. In males E2 measurements may be helpful in the assessment of unexplained gynecomastia. In females E2 measurements are frequently utilized to document hypoestrogenism in cases of delayed puberty, primary and secondary amenorrhea, and menopause. Gonadotropin measurements must be utilized to localize the cause of hypoestrogenism to the ovaries or to a hypothalamic-pituitary source (Perganol(R) induction). E2 concentrations exceeding 1000 pg/ml are usually observed only during pregnancy or in rare cases of estrogen secreting.

Reference Range *

Adult male: < 44 pg/ml; Adult female: early follicular 12-50 pg/ml; late follicular 60-200 pg/ml; midcycle 120-375 pg/ml; luteal phase 50-260 pg/ml; postmenopausal <20 pg/ml.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Levels are reduced by oral contraceptives and estrogen or progesterone therapy.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

4 hours

Soft Order Code
ESTRA
MiChart Code
Estradiol
Synonyms
  • E2
  • Estradiol (E2)
  • Estradiol-17 Beta (E2)
  • Estrogen
  • ESTRA
  • ESTRADIOL
  • ESTRADIOL LEVEL
  • EEST
  • Estradiol, Serum
Laboratory
Chemical Pathology
Section
Automation
STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

Specimen Requirements
Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate. Test is not useful on pregnant women.

Alternate Specimen
Red Top Tube
Normal Volume
0.50 mL serum
Minimum Volume
0.25 mL serum
Additional Information

When total estrogen serum levels are ordered, MLabs will order and perform a serum estradiol level, which is by far the most potent naturally occurring estrogen, almost exclusively produced in the ovaries. As a result, the measurement of serum estradiol is considered sufficient to evaluate ovarian function.

Billing
CPT Code
82670
Fee Code
23328
LOINC
35384-7