Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

This test detects rearrangements involving the ERG gene region (21q22) via fluorescence in situ hybridization (FISH) in formalin-fixed, paraffin-embedded tissue specimens. FISH is performed using a dual-color break-apart probe (Empire Genomics) to qualitatively assess for the presence of ERG rearrangements that are associated with a subset of prostatic adenocarcinoma. Testing for ERG gene rearrangement may aid in: 1. Diagnosis and classification of a subset of prostatic adenocarcinoma 2. Diagnosis and classification of a subset of neuroendocrine prostate cancer 3. Confirmation of presence or absence of ERG gene rearrangement in prostatic adenocarcinoma cases with equivocal ERG immunohistochemical expression. 4. Differential diagnosis of poorly differentiated prostate cancer versus poorly differentiated urothelial cancer 5. Diagnosis of ERG positive metastatic prostatic adenocarcinoma 6. Diagnosis of ERG positive prostatic adenocarcinoma with low or equivocal ERG immunohistochemical expression (for example, treated prostate cancer).

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This test detects rearrangements involving ERG, however it will not identify the translocation partner.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:00pm
Soft Order Code
CGERG
MiChart Code
ERG Rearrangement by FISH (cytogenetics)
Synonyms
  • CGERG
  • ERG gene region 21q22
  • FFPE FISH for ERG
  • Prostatic Adenocarcinoma
  • Prostate Cancer
  • ERG FISH
Laboratory
Cytogenetics
Section
Cytogenetics
Specimen Requirements
Collection Instructions

Submit a formalin-fixed, paraffin block of the tumor tissue (with matched benign prostate tissue if available); store at room temperature.

Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws.

Billing
CPT Code
88271, 88275
Fee Code
EA042, EA043