Polymerase Chain Reaction (PCR)
The QEBV assay is intended to measure EBV viral load in patient plasma to aid in monitoring for, and diagnosis of, post-transplant lymphoproliferative disorder.
Not Detected. The reportable range of the assay is from:
501 I.U./mL (Log10 2.7 I.U./mL) to 10,000,000 I.U./mL (Log10 7.0 I.U./mL)
Virus that is detectable at levels above or below the reportable range will be reported but not quantified. The analytical limit of detection of the assay is 178 (log10 2.25) IU/mL and the precision of the assay is a standard deviation of <0.15 log10 IU/mL throughout the reportable range.
A single assay result should not be used as the only criteria to form a clinical conclusion. Results should be correlated with serologic test results, patient symptoms and clinical presentation.
Performed daily Monday-Saturday. 8 hours analytic time.
- EBV QUANTITATION, PCR
- EBV Viral Load, Plasma
Collect specimen in a 6 ml lavender top (EDTA) tube. Centrifuge for 20 minutes at 800 - 1600 xg at room temperature and aliquot plasma into a polypropylene plastic vial within 24 hours of collection. Refrigerate for up to 5 days or freeze for longer storage. Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the viral load. Microbiology molecular testing requires a dedicated sample, and cannot be shared with other laboratories.