Test Overview
Test Methodology

Multiplex Flow Immunoassay

Test Usage

Support diagnosis of EBV infection.

Reference Range *

NEGATIVE: Indicates no detectable IgG antibody to EBV Nuclear Antigen; EQUIVOCAL: Presence or absence of IgG antibody to EBV Nuclear Antigen cannot be determined. Another specimen should be sent for analysis; POSITIVE: Indicates detectable levels of IgG antibody to EBV Nuclear Antigen.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Assay performance characteristics have not been established for immunocompromised or immunosuppressed patients, cord blood, neonatal specimens or infants. Assay performance characteristics have not been established for the diagnosis of nasopharyngeal carcinoma, Burkitt’s lymphoma and other EBV-associated lymphomas.

Test Details
Days Set Up
Monday - Friday
Analytic Time

24 hours

Soft Order Code
MiChart Code
Epstein-Barr virus nuclear antigen antibody, IgG
  • EBNA
  • EBV Nuclear Ag, IgG
Chemical Pathology
Specimen Requirements
Collection Instructions

Collect specimen in SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate.

Alternate Specimen
Red top tube.
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Rejection Criteria
CSF is not acceptable.
Additional Information

IgG antibodies to EBNA-1 are rarely present in acute IM and normally rise during convalescence. A rise may be indicative of progression from early to later stages of convalescence. The presence of antibodies in otherwise healthy individuals usually indicates past exposure. Antibody levels will rise to a plateau level in three months to a year and will normally persist for life.

CPT Code
Fee Code