Days Set Up
Monday - Friday
Analytic Time

3 - 6 days

Soft Order Code

Update Type: Specimen collection or handling and/or storage changed

Test Updated: 07/22/2020


Biphasic Hemolysin
PCH Screen
PCH Screen
Biphasic Hemolysin

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Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

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MLabs provides all the supplies necessary for the collection of specimens.

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Test Overview

Test Methodology


Test Usage

To rule out the diagnosis of paroxysmal cold hemoglobinuria (PCH) in cases of hemoglobinuria, cold exacerbated hemolysis, unusual presentation of apparent warm or cold AIHA. A positive test is considered diagnostic for PCH.

Reference Range

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect specimen in a red top tube. Maintain at 37 +/- 2 degrees C from the time it is collected until serum is separated from the cells. Within one minute of collection, place specimen into a 37+/- 2 degrees C incubator or waterbath. Leave specimen at 37 degrees C until clot retracts; after the clot retracts, remove serum from clot, centrifuge at room temperature, aliquot 5 mL cell free serum into a plastic tube and refrigerate. Please note on requisition or as a footnote in the computer that the specimen was processed at 37 degrees C.

Normal Volume
3 mL serum
Minimum Volume
1 mL serum

Billing Information

CPT Code
86940, 86941
Pro Fee Code

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.