Test Overview
Test Methodology

Chemiluminescent Immunoassay

Test Usage

In high titer, marker antibody for systemic lupus erythematosus (SLE). Monitor clinical course and response to treatments, especially in patients with nephritis. Anti-dsDNA titers do not correlate with disease activity in all patients.

Reference Range *

<27 IU/mL (Negative); 27 - 35 IU/mL (Borderline Positive); >=36 IU/mL (Positive).

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Varies; test performed 3-4 times per week
Analytic Time

8 hours

Soft Order Code
ADNA
MiChart Code
Anti-DNA Antibody, Double Stranded
Synonyms
  • Anti-DNA Double Stranded (EIA)
  • Anti-ds-DNA
  • Double-Stranded DNA Antibody
  • ds-DNA
  • Native DNA Antibody
  • n-DNA
  • ADNA
  • ANTI-dsDNA ANTIBODY
Laboratory
Chemical Pathology
Section
Special Chemistry
Specimen Requirements
Collection Instructions

Collect specimen in an SST tube. Centrifuge, aliquot serum into a plastic tube and refrigerate for up to 7 days or freeze for longer storage.

Contraindications
Test not usually required on patients with negative ANAs
Yellow Top Tube
Normal Volume
0.75 mL serum
Minimum Volume
0.25 mL serum
Additional Information

Significant elevations are specific for antibodies to Double-Stranded DNA and are highly suggestive of systemic lupus. Weakly positive tests may be due to cross-reactivity with antibody to Single-Stranded DNA or may occassionally be seen in rheumatoid arthritis and other connective tissue diseases. Clinical correlation, including the results of ANA and ENA testing, is important.

Billing
CPT Code
86225
Fee Code
OA003
LOINC
32677-7
Resources