ELISA (Enzyme Linked Immunosorbent Assay)
This assay detects both IgG and IgM antibodies against all four Dengue fever virus types. Except for very early IgM responses, the immune response to Dengue fever is not type specific. Therefore, type specific reactions are not reported. As with most serological assays paired testing of acute and convalescent samples is preferred. This is especially important when the acute phase sample is taken within the six days following onset. In most patients, Dengue antibodies are detectable after the sixth day following the onset of symptoms.
IgG: Negative, IgM: Negative
False-positive results, particularly with the dengue virus (DV) IgG enzyme-linked immunosorbent assay (ELISA), may occur in persons infected with other flaviviruses, including West Nile virus and St. Louis Encephalitis virus. Obtaining a detailed exposure history and further laboratory testing may be necessary to determine the infecting virus.
1 - 4 days
Collect specimen in a red top or SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate.
Test sent to Mayo Medical Laboratories.