This assay detects both IgG and IgM antibodies against all four Dengue fever virus types. Except for very early IgM responses, the immune response to Dengue fever is not type specific. Therefore, type specific reactions are not reported. As with most serological assays paired testing of acute and convalescent samples is preferred. This is especially important when the acute phase sample is taken within the six days following onset. In most patients, Dengue antibodies are detectable after the sixth day following the onset of symptoms.