Test Overview
Test Methodology

Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS)

Test Usage

DHEA is the primary adrenal androgen and is an important precursor in the biosynthesis of testosterone and estrogens. Nevertheless, circulating DHEA does not appear to be a major precursor of testosterone in cases of androgen excess. It has also been shown that FREE DHEA may be derived in large part from the hydrolysis of circulating DHEA-sulfate, the most abundant adrenal steroid known. Free DHEA levels are often used in conjunction with plasma testosterone, androstenedione, and DHEA-sulfate. Measurement of DHEA using this assay is useful for diagnosis of disorders related to adrenal androgen production including (1) adrenal hyperplasia (2) adrenal tumors (3) adrenarche (4) delayed puberty and (5) hirsutism.

Reference Range *

*Ranges provided by Chemical Pathology, Special Testing Section

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

DHEA levels are elevated by estrogens, aldactone, and during pregnancy.

Test Details
Days Set Up
Analytic Time

8 hours

Soft Order Code
MiChart Code
DHEA (Dehydroepiandrosterone)
  • DHEA
  • Free DHEA
Chemical Pathology
Drug Analysis
Specimen Requirements
Collection Instructions

Collect specimen in a red top (preferred) or SST tube. Centrifuge, aliquot serum into a plastic vial and freeze.

Normal Volume
1 mL serum
Minimum Volume
0.6 mL serum
Additional Information

Increased serum levels of DHEA may be associated with malignant neoplasm of the adrenal gland (virilizing adrenal tumors) and Stein-Leventhal syndrome. DHEA also may be increased in serum with administration of clomiphene and corticotropin. DHEA in the urine may be decreased due to administration of Dexamethasone (suppression of ACTH) and oral contraceptives.

CPT Code
Fee Code