Days Set Up
Monday – Thursday
Analytic Time

3 – 10 days

MiChart Code
DDIT3 (12q13) Rearrangement by FISH
Soft Order Code
MDDIT

Update Type: New Tests

Test Updated: 01/13/2021

Synonyms
 

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Test Overview

Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

DDIT3 (formerly CHOP) encodes a transcription factor involved in adipogenesis and erythropoiesis. Rearrangements involving this gene are characteristic of myxoid liposarcoma. Morphologically, myxoid liposarcomas demonstrates uniform, small spindled cells within a myxoid stroma with distinctive arborizing capillaries. A subset of myxoid liposarsomas show progression to round cell morphology, associated with a poorer prognosis. Approximately 95% of myxoid liposarcomas bear a t(12;16)(q13;p11) rearrangement that results in the fusion of the N-terminal transactivation domain of FUS and the full length of DDIT3. In the remaining cases, there is a similar rearrangement involving DDIT3 and EWSR1. The detection of DDIT3 rearrangements can be useful in diagnosing myxoid liposarcoma – including uncommon histologic variants – and in distinguishing this sarcoma from other mesenchymal tumors that may be considered in the differential diagnosis.

Reference Range
 

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

A formalin-fixed, paraffin-embedded tissue block (containing sufficient neoplastic cells) is preferred. Unstained slides (3 slides cut at 4-microns) with associated H&E-stained slide are also acceptable. Decalcified tissue or tissues with other fixatives will be accepted and the assay attempted; however, these specimens may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Collection Offsite

A formalin-fixed, paraffin-embedded tissue block (containing sufficient neoplastic cells) is preferred. Unstained slides (3 slides cut at 4-microns) with associated H&E-stained slide are also acceptable. Decalcified tissue or tissues with other fixatives will be accepted and the assay attempted; however, these specimens may result in failed testing due to degraded nucleic acid. Both blocks and slides should be stored at room temperature.

Normal Volume
Formalin-fixed, paraffin-embedded tissue
Minimum Volume
 

Billing Information

CPT Code
88377-TC
Pro Fee Code
 
LOINC
 

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Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.