Test Overview
Test Methodology

Anti-IIa clottable assay. This assay is able to detect dabigatran levels between 50 - 1000 ng/ml.

Test Usage

Used for determining dabigatran anti-IIa activity in plasma.

Reference Range *

Normal individuals have no dabigitran activity. A correlation of anti-IIa activity and therapeutic benefit has not been demonstrated. If this test result is greater than 50 ng/mL, then the patient has some detectable anticoagulant effect from dabigatran.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

2 hours

Soft Order Code
MiChart Code
Dabigatran Level, Anti-IIA Inhibitor Assay
  • Ecarin Clotting Time
  • Dabigatran Etexilate
  • Pradaxa
  • Anti-IIa Inhibitor Assay
STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

Specimen Requirements
Collection Instructions

Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 4 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

Not to be used for patients receiving argatroban therapy.
Normal Volume
Full 2.7mL tube or 1mL plasma aliquot
Minimum Volume
Full 1.8mL tube or 1mL plasma aliquot
Storage Temperature
Room temperature for whole blood or frozen aliquot of citrated plasma
Rejection Criteria
Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, contaminated with heparin, or received outside acceptable time limits.
CPT Code
Fee Code