Test Overview
Test Methodology

Real-Time Polymerase Chain Reaction (PCR)

Test Usage

Measures the CMV viral load present at the time of specimen collection. Used as an aid in determining or altering treatment in CMV infected, immunocompromised patients.

Reference Range *

Not detected in uninfected patients. Patients with latent infection may have a detectable viral load at baseline.

Test Limitations

A single assay result should not be used as the only criteria to form a clinical conclusion. Results should be correlated with serologic test results, patient symptoms and clinical presentation.

Test Details
Days Set Up
Tuesday - Saturday
Analytic Time

8 hours

Soft Order Code
MiChart Code
CMV DNA,Quantitative
  • CMV Antigenemia Replacement Assay
  • CMV DNA Quantitation
  • CMV pp65
  • CMV Viral Load
  • Cytomegalovirus Viral Load
  • pp65 Antigen
  • Viral Load, Cytomegalovirus (CMV)
  • QCMV
Specimen Requirements
Collection Instructions

Collect specimen in a lavender top (EDTA) tube. Centrifuge for 20 minutes at 800 - 1600 xg at room temperature and aliquot plasma into a polypropylene plastic vial within 24 hours of collection. Refrigerate for up to 5 days or freeze for longer storage. Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the DNA. Specimens submitted in polystyrene tubes are not acceptable. Specimens sent for Microbiology molecular testing require a dedicated sample and cannot be shared with other laboratories.

Normal Volume
4 mL whole blood or 2 mL plasma
Minimum Volume
2 mL whole blood or 1 mL plasma
Storage Temperature
Specimen can be held up to 24 hours prior to centrifugation at 2-8 C.Post centrifugation plasma aliquot may be held at 15- 30 C for up to 24 hours, held at 2-8 C for up to 5 days, or at -70 C for longer.
Rejection Criteria
Specimens received in polystyrene aliquot tubes are unacceptable and will be rejected. Plasma must be removed from cells in 24 hours of collection.
Additional Information

The lower limit of detection is 31.2 IU/mL (1.49 log IU/mL). The quantitative range of this assay is 50 IU/mL to 156,000,000 IU/mL (1.70 to 8.19 LOG10) at the lower limit of quantification, the difference between two measurements of 1 log IU/mL (10 fold) is statistically significant. Virus present below 50 IU/ mL will be reported as detected but not quantified. If results are greater than 156,000,000 IU/mL a dilution of the sample will be run for an exact quantitation. For Mlabs, please contact client service center if actual viral load is needed.

CPT Code
Fee Code
NY State Approved