Plasma cell (CD138) enrichment
Interphase Fluorescence In Situ Hybridization (FISH)
Multiple myeloma FISH panel aids in stratifying individuals with newly diagnosed multiple myeloma into risk groups for prognosis and selection of therapy. It is also useful in following up remission or relapse status.
For diagnostic samples, the test will be run in a tiered approach. The first tier tests include 17p/cen (TP53/CEP17), t(4;14) (IGH/FGFR3), 14q (IGH), and 1p/1q (CKS1B/CDKN2C). Second tier probes are, +5 (D5S23, D5S721), +9 (CEP 9), +15 (CEP15), 8q24 (MYC), and del(13q)/-13 (D13S319/13q34). When an IGH rearrangement is identified and the partner is not FGFR3, reflex testing is performed to identify the translocation partner. Probes include identification of t(11;14) CCND1/IGH, t(14;16) IGH/MAF, and t(14;20) IGH/MAFB.
For follow-up samples, probes that were abnormal in a previous study will be tested along with 1p/1q, TP53/cen17 and MYC probes if sufficient plasma cells are obtained. If previous FISH results were unknown, the sample will be evaluated as a diagnostic sample.
Interpretive report provided.
For diagnostic samples, the results of t(4;14) and 17p (TP53) will be provided in a preliminary report within 7 days. The final report will be completed in 14 days.
- FISH for Multiple Myeloma
- Plasma Cell Proliferative Disorder (PCPD), FISH
- FISH for Plasma Cell Disorder
Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. The sample must be in the Cytogenetics lab Monday - Friday by 4pm. Call for a STAT courier if necessary. Collect specimen in a green top (sodium heparin) tube. Invert the tube several times to prevent clotting. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Clotted samples or those that are greater than 24 hours old are suboptimal. Multiple Myeloma FISH panel testing may be performed as a "stand alone" test or included with Chromosome Analysis.
"Stand alone" FISH testing will include Tissue Culture at an additional charge. Appropriate additional FISH testing will be performed at an additional charge when indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.