Days Set Up
Monday - Friday, 8:00am - 4:00pm
Analytic Time

7-10 days

MiChart Code
Cytogenetics, High-grade B-cell Lymphoma FISH Panel
Soft Order Code
CGLMP

Test Updated:

Synonyms
 

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All specimens should be accompanied by a requisition.

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MLabs provides all the supplies necessary for the collection of specimens.

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Test Overview

Test Methodology

Interphase Fluorescence In Situ Hybridization (FISH)

Test Usage

This panel differentiates double-hit or triple-hit lymphomas (which have MYC rearrangements together with BCL2 and/or BCL6 rearrangements) from Burkitt lymphoma or diffuse large B-cell lymphoma. Double-hit and triple-hit lymphomas are difficult to classify morphologically without the aid of cytogenetics/FISH or IHC, and are associated with an aggressive course. Testing is indicated when B-cell lymphoma patients experience transformation, relapse, or refractory disease. MYC rearranges with an immunoglobulin partner in approximately 60% of MYC-rearranged DLBCL/HGBCL of which 75% are MYC/IGH fusion. In addition, a small subset of lymphomas with MYC rearrangement (4%) may be missed when using the MYC breakapart probe alone, but some of these cases may be detected using the MYC/IGH fusion probe set (PMID: 30523057). The FISH panel for high grade B-cell lymphoma includes MYC (8q24), IGH/MYC/CEN8 [t(8;14)], BCL2 (18q21) and BCL6 (3q27) translocations.

Reference Range

Interpretive report provided.
*Reference ranges may change over time. Please refer to the original patient report when evaluating results.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Onsite

Collect specimen in a sterile syringe rinsed with sodium heparin or a green top vacutainer (sodium heparin) tube. Invert several times to prevent clotting. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Please complete a MiChart test request and submit it with the specimen. A Cytogenetics Laboratory Requisition is available online at:http://www.pathology.med.umich.edu/handbook/Tables/UM_REQ_CYTOGENETICS….

Collection Offsite

Collect blood or bone marrow using aseptic technique. Place 2-5 mL blood or 1 - 3 mL bone marrow aspirate in a green top (sodium heparin) tube. Invert the tube several times to prevent clotting. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Clotted samples, samples containing <5 million dividing cells, and samples that are greater than 24 hours old are suboptimal.

Normal Volume
5 mL whole blood or 2-3 mL bone marrow
Minimum Volume
2 mL whole blood or 1 mL bone marrow; additional specimen may be required for patients with extremely low white cell counts.

Billing Information

CPT Code
88237x1, 88275 x4, 88271x8
Pro Fee Code
 
LOINC
 

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.