Test Overview
Test Methodology

Fluorescence In Situ Hybridization (FISH)

Test Usage

The ALL FISH Panel is used for the detection of recurrent chromosome abnormalities observed in infants and children with B-cell lymphoblastic leukemia/lymphoma. Identification of specific abnormalities helps predict disease aggressiveness and response to therapy. The B-ALL FISH panel includes: 1) ETV6/RUNX1 probe, to detect t(12;21); this translocation is often cryptic by chromosome analysis. 2) BCR/ABL + ASS dual-fusion probe, to detect t(9;22) and variants. 3) KMT2A (MLL) break-apart probe, to detect KMT2A (11q23.3) rearrangement; the specific rearrangement partner chromosome is not interrogated by FISH. 4-5) CEP 4/CEP 10 enumeration probes, to detect hyperdiploidy involving trisomies of chromosomes 4 and 10. Reflex testing for Xp22.3/Yp11.3 rearrangement (CRLF2) and/or 9p24 rearrangement (JAK2) may be performed after Chromosome Analysis and Chromosomal Microarray Analysis testing, if clinically indicated.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:00pm
Soft Order Code
MiChart Code
Cytogenetics, ALL FISH Panel
Specimen Requirements
Collection Instructions

ALL FISH testing is performed as an adjunct to Chromosome Analysis when appropriate using the specimen submitted for Chromosome Analysis, unless otherwise approved by the Cytogenetics Laboratory Director. Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Call for a STAT courier if necessary. See "Cytogenetics, Chromosome Analysis, Bone Marrow" or "Cytogenetics, Chromosome Analysis, Blood for Malignancy" for specimen collection and handling requirements.

Normal Volume
5 mL whole blood or 2 mL bone marrow
Minimum Volume
2 mL whole blood or bone marrow; additional specimen may be required for patients with extremely low white cell counts or on COG study.
Rejection Criteria
Clotted or hemolyzed specimen, specimen over 24 hours old, or those from septic or transfused patients.
Additional Information

"Stand alone" FISH testing will include Tissue Culture at an additional charge. Appropriate additional FISH testing will be performed at an additional charge when indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
88237, 88271 x5, 88275 x7
Fee Code
36542, EA040 x5, EA041 x7