Fluorescence In Situ Hybridization (FISH)
The ALL FISH Panel is used for the detection of recurrent chromosome abnormalities observed in infants and children with B-cell lymphoblastic leukemia/lymphoma. Identification of specific abnormalities helps predict disease aggressiveness and response to therapy. The B-ALL FISH panel includes: 1) ETV6/RUNX1 probe, to detect t(12;21); this translocation is often cryptic by chromosome analysis. 2) BCR/ABL + ASS dual-fusion probe, to detect t(9;22) and variants. 3) MLL break-apart probe, to detect MLL (11q23.3) rearrangement; the specific rearrangement partner chromosome is not interrogated by FISH. 4-6) CEP 4/CEP 10/ CEP 17 enumeration probes, to detect hyperdiploidy involving trisomies of chromosomes 4, 10 and 17. Reflex testing for Xp22.3/Yp11.3 rearrangement (CRLF2) and/or 9p24 rearrangement (JAK2) may be performed after Chromosome Analysis and Cancer Cytogenomics Array testing, if clinically indicated.
Interpretive report provided.
ALL FISH testing is performed as an adjunct to Chromosome Analysis when appropriate using the specimen submitted for Chromosome Analysis, unless otherwise approved by the Cytogenetics Laboratory Director. Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Call for a STAT courier if necessary. See "Cytogenetics, Chromosome Analysis, Bone Marrow" or "Cytogenetics, Chromosome Analysis, Blood for Malignancy" for specimen collection and handling requirements.
"Stand alone" FISH testing will include Tissue Culture at an additional charge. Appropriate additional FISH testing will be performed at an additional charge when indicated. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.