Test Overview
Short term cell culture and chromosome analysis, cultures with and without mitogens. Fluorescence In Situ Hybridization (FISH) when indicated. Samples with an indication of mature B-cell lymphoma including, but not limited to, CLL/SLL, follicular lymphoma, mantle cell lymphoma and marginal zone lymphoma, will have an extra culture inoculated with CpG-oligonucleotide (CpG) and Interleukin-2 (IL2). A minimum of 10 metaphase cells are analyzed from mitogen stimulated cultures, in addition to the standard 20 metaphase cells analyzed from the unstimulated cultures.
Identification of chromosomal abnormalities to assist in classification and prognostication of lymphomas. Most lymphomas are associated with genetic changes and the observation of an abnormal cytogenetic clone is consistent with a neoplasm.
Interpretive report provided.
* Reference ranges may change over time. Please refer to the original patient report when evaluating results.
Cells must be capable of dividing. Necrotic specimens of those older than 24 hours may not grow. Specimens collected using non-sterile techniques may have microbial contamination.
Test Details
7-10 days
- Karyotype, Mass
- Karyotype, Neoplasm
- Cytogenetics, Lymphoma
- Chromosome Analysis, Lymph Node for Malignancy
Specimen Requirements
Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Collect tissue sample using aseptic technique. Place tissue in RPMI 1640, available from MLabs. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Small or necrotic samples, samples obtained without collection media, and samples that are greater than 24 hours old are considered suboptimal.
Additional Information
Appropriate FISH testing will be performed at an additional charge when indicated. FISH testing is available for a number of oncology probes, including FISH panel for High-grade B-cell lymphoma. For additional information on FISH testing and available probes, contact the Cytogenetics Laboratory. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.