Test Overview
Test Methodology

Short term cell culture and chromosome analysis, cultures with and without mitogens. Fluorescence In Situ Hybridization (FISH) when indicated. Samples with an indication of mature B-cell lymphoma including, but not limited to, CLL/SLL, follicular lymphoma, mantle cell lymphoma and marginal zone lymphoma, will have an extra culture inoculated with CpG-oligonucleotide (CpG) and Interleukin-2 (IL2). A minimum of 10 metaphase cells are analyzed from mitogen stimulated cultures, in addition to the standard 20 metaphase cells analyzed from the unstimulated cultures.

Test Usage

Identification of chromosomal abnormalities to assist in classification and prognostication of lymphomas. Most lymphomas are associated with genetic changes and the observation of an abnormal cytogenetic clone is consistent with a neoplasm.

Reference Range *

Interpretive report provided.

Test Limitations

Cells must be capable of dividing. Necrotic specimens of those older than 24 hours may not grow. Specimens collected using non-sterile techniques may have microbial contamination.

Test Details
Days Set Up
Monday - Friday, 8:00 am - 4:00 pm. Call the laboratory at (734) 763-5805 for additional information.
Analytic Time

7-10 days

Soft Order Code
MiChart Code
Cytogenetics, Chromosome Analysis/Karyotype, Lymphoma Work-Up
  • Karyotype, Mass
  • Karyotype, Neoplasm
  • Cytogenetics, Lymphoma
  • Chromosome Analysis, Lymph Node for Malignancy
Specimen Requirements
Collection Instructions

Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Collect tissue sample using aseptic technique. Place tissue in Chromosome Media, available from MLabs. Send at room temperature as soon as possible. Do not allow specimen to overheat or freeze. Small or necrotic samples, samples obtained without collection media, and samples that are greater than 24 hours old are considered suboptimal.

Normal Volume
3 cm(3) or larger
Minimum Volume
0.5 cm(3)
Rejection Criteria
Formalin fixed, frozen or grossly contaminated specimens.
Additional Information

Appropriate FISH testing will be performed at an additional charge when indicated. FISH testing is available for a number of oncology probes, including FISH panel for High-grade B-cell lymphoma. For additional information on FISH testing and available probes, contact the Cytogenetics Laboratory. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
88262, 88239, 88280x3, 88285
Fee Code
36543, EA001, EA030x3, EA031, 36552
NY State Approved