Test Overview
Test Methodology

Short term cell culture and chromosome analysis, cultures with and without mitogens. Fluorescence In Situ Hybridization (FISH) when indicated. Samples with an indication of mature B-cell lymphoma including, but not limited to, CLL/SLL, follicular lymphoma, mantle cell lymphoma and marginal zone lymphoma, will have an extra culture inoculated with CpG-oligonucleotide (CpG) and Interleukin-2 (IL2). Similarly, samples with an indication of mature T-cell lymphoma/leukemia (T-PLL, AITCL, ALCL) will have an extra culture inoculated with Phytohemagglutinin (PHA). A minimum of 10 metaphase cells are analyzed from mitogen stimulated cultures, in addition to the standard 20 metaphase cells analyzed from the unstimulated cultures.

Test Usage

Identification of specific chromosomal abnormalities associated with hematologic disease. Bone Marrow specimens are most commonly sent as part of the evaluation of acute leukemia (lymphoid or myeloid), MDS, or myeloproliferative disorders including chronic myelogenous leukemia, in the latter case to look for a Philadelphia chromosome.

Reference Range *

Interpretive report provided.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Cells must be capable of dividing.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:00pm. Call the laboratory at 734-763-5805 for additional information.
Analytic Time

7-10 days

Soft Order Code
MiChart Code
Cytogenetics, Chromosome Analysis/Karyotype, Bone Marrow
  • Cytogenetics, Bone Marrow
  • Karyotype, Bone Marrow
  • Chromosome Analysis, Bone Marrow for Malignancy
  • TCBM
  • Chromosome Analysis for Hematologic Disorders, Bone Marrow
Specimen Requirements
Collection Instructions

Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Collect bone marrow using aseptic technique. Place 1 - 3 mL bone marrow aspirate in a green top (sodium heparin) tube. Invert the tube several times to prevent clotting. Send at room temperature as soon as possible. Bone core biopsies must be transported in sterile transport media. Do not allow specimen to overheat or freeze. Clotted samples, samples containing <5 million dividing cells, and samples that are greater than 24 hours old are considered suboptimal.

Normal Volume
2-3 mL bone marrow
Minimum Volume
1 mL bone marrow
Rejection Criteria
Formalin fixed, frozen, or grossly contaminated specimens.
Additional Information

Appropriate FISH testing will be performed at an additional charge when indicated. FISH testing is available for a number of oncology probes, including BCR/ABL, PML/RARA, ETV6/RUNX1, and KMT2A and MDS FISH panel (see Cytogenetics, FISH for Malignancy). For additional information on FISH testing and available probes, contact the Cytogenetics Laboratory. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
88264, 88237, if applicable: 88280 x3, 88285 x2
Fee Code
EA038, 36542, if applicable: EA030 x3, EA031, 36552