Test Overview
Test Methodology

Short term cell culture and chromosome analysis, cultures with and without mitogens. Fluorescence In Situ Hybridization (FISH) when indicated. Samples with an indication of mature B-cell lymphoma including, but not limited to, CLL/SLL, follicular lymphoma, mantle cell lymphoma and marginal zone lymphoma, will have an extra culture inoculated with CpG-oligonucleotide (CpG) and Interleukin-2 (IL2). Similarly, samples with an indication of mature T-cell lymphoma/leukemia (T-PLL, AITCL, ALCL) will have an extra culture inoculated with Phytohemagglutinin (PHA). A minimum of 10 metaphase cells are analyzed from mitogen stimulated cultures, in addition to the standard 20 metaphase cells analyzed from the unstimulated cultures.

Test Usage

Rule out acquired chromosomal abnormalities associated with hematologic disease. If the patient has at least 10% blasts (or a combination of blasts, promyelocytes and myelocytes totaling 10%) a peripheral blood sample may be useful, however, a bone marrow sample is preferred.

Reference Range *

Interpretive report provided.

Test Limitations

Neoplastic cells not dividing or not circulating in the bloodstream.

Test Details
Days Set Up
Monday - Friday, 8:00am - 4:00pm. Call the laboratory at 734-763-5805 for additional information.
Analytic Time

7-10 days

Soft Order Code
MiChart Code
Cytogenetics, Chromosome Analysis, Blood for Malignancy
  • Chromosome Analysis, Blood for Malignancy
  • Karyotype, Malignancy
  • Chromosome Analysis for Hematologic Disorders, Blood
Specimen Requirements
Collection Instructions

Specimen transport should be arranged so that the specimen is received by MLabs the same day it is collected. Collect specimen in a green top (sodium heparin) tube. Invert the tube several times to prevent clotting. Send the specimen at room temperature as soon as possible. DO NOT CENTRIFUGE. Clotted samples, samples containing <5 million dividing cells, and samples that are greater than 24 hours old are considered suboptimal.

Rejection Criteria
Formalin fixed, frozen or grossly contaminated specimens.
Patient receiving antileukemic drugs, no circulating immature cells capable of division.
Normal Volume
5-10 mL whole blood
Minimum Volume
2 mL whole blood; additional specimen may be required for patients with extremely low white cell counts.
Additional Information

Appropriate FISH testing will be performed at an additional charge when indicated. FISH testing is available for a number of oncology probes, including BCR/ABL, PML/RARA, ETV6/RUNX1, and KMT2A and MDS FISH panel (see Cytogenetics, FISH for Malignancy). For additional information on FISH testing and available probes, contact the Cytogenetics Laboratory. By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
88262, 88237, 88280x3, 88285
Fee Code
36526, 36542, EA030x3, EA031, 36552
NY State Approved