Test Overview
Test Methodology

Chemiluminescent Immunoassay (CLIA)

Test Usage

Evaluation of patients with possible myocardial infarction. Following myocardial infarction, CK-MB levels generally start to rise after 4-6 hours, peak around 20 hours, and return to normal by 48 hours. Thrombolytic therapy can shorten these times considerably.

Reference Range *

MB Isoenzyme: 0 - 4.9 ng/mL (if total CK <500 IU/L) or 0.0 - 1.0% (if total CK >=500 IU/L).

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

2 hours

Soft Order Code
MiChart Code
CK Total and CKMB (TCK)
  • CKMB %
  • CK Total and MB Isoenzyme
  • CPK-MB
  • Creatine Kinase Fractionation
  • Creatine Phosphokinase Isoenzyme
  • Total CPK
  • TCK
  • CKMB%
  • CKMB
  • CK Isoenzymes, MB
Chemical Pathology
STAT Availability

STAT requests for this test will be performed on a STAT basis (supervisory staff approval is not required).

Specimen Requirements
Collection Instructions

Collect specimen in a light green top plasma separator tube. Centrifuge, aliquot plasma into a plastic vial and refrigerate.

Alternate Specimen
Green (lithium or heparin), SST or Red top tube. Light green plasma separator tube preferred if troponin T testing ordered. Serial specimens should be collected in the same container type. [rev 12/09; 3/11; 5/19]
Normal Volume
0.5 mL plasma
Minimum Volume
0.4 mL plasma
Additional Information

The test includes a total CK determination. CK-MB will not be run if total CK < 80 IU/L. If total CK is > or = 80 IU/L and < 500 IU/L, the CKMB will be performed (reference range 0 - 4.9 ng/mL). If total CK is > or = 500 IU/L, the CKMB (no reference range) and CKMB% (reference range 0.0 - 1.0%) will be performed.

CPT Code
82550 CK, 82553 CK-MB
Fee Code
20712 CK, 20908 CK-MB
CKMB 13969-1