Days Set Up
Monday - Thursday
Analytic Time

2 days

MiChart Code
Cortisol/Cortisone, Free, Urine
Soft Order Code

Test Updated:


Cortisol, Free
Free Cortisol
Urinary Free Cortisol
Urine Cortisol

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Test Overview

Test Methodology


Test Usage

Very helpful in evaluating suspected Cushing's syndrome. This test is specific for uncovering elevated urinary unconjugated cortisol. Low values may suggest adrenal insufficiency.

Reference Range

CORTISOL: 0-2 years: not established, 3-8 years: 1.4 - 20.0 mcg/24hours, 9-12 years: 2.6 - 37.0 mcg/24 hours, 13-17 years: 4.0 - 56.0 mcg/24 hours, 18-150 years: 3.5 - 45.0 mcg/24 hours.
CORTISONE: 0-2 years: not established, 3-8 years: 5.5 - 41.0 mcg/24hours, 9-12 years: 9.9 - 73.0 mcg/24 hours, 13-17 years: 15 - 108 mcg/24 hours, 18-150 years: 17 - 129 mcg/24 hours.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Specimen Requirements

Collection Offsite

Collect 24 hour urine specimen with no preservative. Aliquot 5 ml into a plastic tube and refrigerate. Record total 24 hour urine volume and collection dates/times on request form. Treatment with hydrocortisol should be discontinued at least 2 days prior to this test.

Normal Volume
5 ml aliquot of 24 hour urine collection
Minimum Volume
3 ml aliquot of 24 hour urine collection

Billing Information

CPT Code
Pro Fee Code
2147-7 (Urine Free Cortisol mcg/24 hours)

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Our High Standard

Quality that sets us apart

As the reference laboratory division of Michigan Medicine's Department of Pathology, MLabs shares the institution's commitment to applying established quality principles to clinical laboratory testing. Like other large organizations in complex, consequential fields, we rely on an established approach to monitor quality throughout the testing process.