Test Overview
Test Methodology

Chemiluminescent Immunoassay (CLIA)

Test Usage

Establish diagnosis of adrenocortical insufficiency, Addison's disease, adrenocortical hypersecretion, or Cushing's disease. Malfunction of any of the organs in the hypothalamus - pituitary - adrenal cortex axis will result in alteration of cortisol levels.

Reference Range *

AM: 7.0 - 22.0 mcg/dL; PM: 3.0 - 13.0 mcg/dL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Specimen may be stored at room temperature up to 8 hours.

Test Details
Days Set Up
Daily, 24 hours
Analytic Time

4 hours

Soft Order Code
CORT
MiChart Code
Cortisol
Synonyms
  • CORTISOL LEVEL
  • Hydrocortisone
  • CORT
  • CORTISOL
  • CORTI
  • Cortisol, IVC
  • CORTR
  • Cortisol, Right IVC
  • CORTL
  • Cortisol, Left IVC
  • Dexamethasone Suppression Test
Laboratory
Chemical Pathology
Section
Automation
Specimen Requirements
Collection Instructions

Collect specimen in a red top or SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate up to 48 hours or freeze for longer storage. Circulating cortisol levels follow a diurnal pattern. Cortisol values are the highest in the morning and lowest in the evening. A single analysis of serum cortisol is of little diagnostic value because of daily variation. Blood should be drawn in the morning and late afternoon to evaluate baseline diurnal variation. Indicate time and date of draw on requisition.

Alternate Specimen
Red Top Tube
Rejection Criteria
Anticoagulants not acceptable.
Yellow Top Tube
Normal Volume
0.5 mL serum
Minimum Volume
0.25 mL serum
Billing
CPT Code
82533
Fee Code
23301
LOINC
57862-5
Resources