Test Overview
Test Methodology

Enzyme-Linked Immunosorbent Assay (ELISA)

Test Usage

The AH50 assay is a useful screening tool for homozygous deficiencies of Alternative Pathway components including C3, factor I, factor B, properdin, factor H and factor D.

Reference Range *

> or = 46% of normal

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Analytic Time

2 - 7 days

Soft Order Code
AH50
MiChart Code
Complement, Total Alternative Path (AH50)
Synonyms
  • Complement AH50
  • AH50
Laboratory
Sendout
Reference Laboratory
Mayo AH50 (88676)
Section
Special Testing
Specimen Requirements
Collection Instructions

A fasting specimen is preferred but not required. Collect specimen in an SST (preferred) or red top tube. Immediately after drawing specimen, place the tube on wet ice and allow specimen to clot. Centrifuge at 4 degrees C, aliquot serum into a plastic vial and freeze within 30 minutes of collection. (Note: if a refrigerated centrifuge is not available, it is acceptable to use a room temperature centrifuge, provided the sample is kept on ice before centrifugation, and immediately afterward, the serum is aliquoted and frozen).

Special Handling

Place sample on wet ice following collection. Must arrive in lab or aliquot and freeze within 30 minutes of collection.

Fasting Specimen
A fasting specimen is preferred but not required.
Normal Volume
1 mL serum
Minimum Volume
0.75 mL serum
Storage Temperature
Strict Frozen: 14 days.
Rejection Criteria
Not collected and placed on ice
Additional Information

Test sent to Mayo Medical Laboratories.

Billing
CPT Code
86161
Fee Code
20493
LOINC
13087-2
NY State Approved
No