Test Overview
Test Methodology

Photo-optical clot determination with Factor II deficient substrate.

Test Usage

Detection of specific coagulation factor II deficiency

Reference Range *

50-150%

Test Limitations

Increased in patients taking oral contraceptives. Interpretation of results may be limited if patient is receiving anticoagulant therapy.

Test Details
Days Set Up
Monday - Friday, one run per day
Analytic Time

1 hour

Soft Order Code
F2
MiChart Code
Factor 2 Assay
Synonyms
  • Factor II Assay
  • Factor II:C Assay
  • F2
  • Factor 2 Assay
  • Factor 2 Activity
Laboratory
Hematopathology
Section
Coagulation
Specimen Requirements
Collection Instructions

Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 3 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

Rejection Criteria
Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, or contaminated with heparin.
Blue Top Tube
Normal Volume
Full 2.7mL tube or 1mL plasma aliquot
Minimum Volume
Full 1.8mL tube or 1mL plasma aliquot
Storage Temperature
Room temperature for whole blood or frozen aliquot of citrated plasma.
Additional Information

Factor II is decreased with vitamin K deficiency, liver disease, during warfarin therapy, and in association with lupus anticoagulant.

Billing
CPT Code
85210
Fee Code
31128
NY State Approved
No