Test Overview
Test Methodology

Photo-optical clot determination with Factor XII deficient substrate.

Test Usage

Detection of specific coagulation factor XII deficiency

Reference Range *

50-150% of normal activity.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday, one run per day
Analytic Time

1 hour

Soft Order Code
F12
MiChart Code
Factor 12 Assay
Synonyms
  • Factor XII Assay
  • Factor XII:C Assay
  • F12
  • Factor 12 Activity
  • Factor 12 Assay
Laboratory
Hematopathology
Section
Coagulation
Specimen Requirements
Collection Instructions

Collect specimen in a blue top (citrate 3.2%) tube. Mix by inversion. Specimen should arrive at lab within 3 hours of collection; transport at room temperature. Alternatively, centrifuge, aliquot plasma into a plastic vial, and freeze the specimen within 4 hours of collection. Transport frozen specimen on dry ice. Collection of the blood through lines that have been previously flushed with heparin should be avoided. If the blood must be drawn through a VAD (vascular access device), the line should be flushed with 5 mL of saline and the first 5 mL of blood or six dead space volumes of the VAD discarded.

Contraindications
Test is not indicated if the patient is receiving heparin or direct thrombin inhibitor anticoagulation therapy.
Normal Volume
Full 2.7mL tube or 1mL plasma aliquot
Minimum Volume
Full 1.8mL tube or 1mL plasma aliquot
Storage Temperature
Room temperature for whole blood or frozen aliquot of citrated plasma.
Rejection Criteria
Specimens will be rejected if not properly filled, clotted, grossly hemolyzed, or contaminated with heparin.
Additional Information

Deficiencies have no hemorrhagic consequence. Factor XII may be increased in patients with pre-eclampsia and decreased in patients with liver disease, renal disease, and Rocky Mountain spotted fever. Congenital Factor XII deficiency has been associated with an increased incidence of venous thrombosis.

Billing
CPT Code
85280
Fee Code
31120
LOINC
3232-6