Test Overview
Test Methodology

Micro-Immunofluorescent Antibody (MIF) Assay

Test Usage

Aiding in the clinical diagnosis of Chlamydia pneumoniae or Chlamydia psittaci infection

Reference Range *

Chlamydia pneumoniae IgM: <1:10 IgG: <1:64; Chlamydia psittaci IgM: <1:10, IgG: <1:64

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

This assay does not report antibodies detected against Chlamydia trachomatis. Sera from suspected cases of lymphogranuloma venereum (LGV) should be tested by a Lymphogranuloma Venereum Differentiation Antibody Panel. LGV testing is not performed by Mayo Clinic Laboratories; call 800-533-1710 for assistance. Due to the limited sensitivity and specificity of Chlamydia serologic tests, patients with suspected C trachomatis infection should be tested by a molecular method (eg, CTRNA / Chlamydia trachomatis, Nucleic Acid Amplification, Varies) when clinical manifestations are present.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 - 5 days

Soft Order Code
Reference Laboratory
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in an SST or red top tube. Spin and aliquot into a screw capped plastic tube. Send to Specimen Processing refrigerated

Antichlamydial IgG can persist for years. All results from chlamydial serologies must correlate with clinical history and other data available to the physician.
• Specimens collected too early during primary infection may not contain detectable antibodies. If chlamydial infection is suspected, a second specimen should be collected 10 to 21 days later and tested in parallel with the original specimen.
• During a primary Chlamydia infection, the early antibody response may be cross-reactive with multiple
Chlamydia species.
Normal Volume
0.6 mL serum
Minimum Volume
0.3 mL serum
Storage Temperature
Refrigerated preferred, frozen acceptable
Additional Information

Test performed by Mayo Clinic Laboratories

CPT Code
86631 x2 IgG, 86632 x2 IgM
Fee Code
AB105 x2, AB106 x2