Test Overview
Test Methodology

Serum IgA (turbidimetric); Transglutaminase IgA/IgG Antibody (Multiplex Flow Immunoassay); Deamidated Gliadin IgA/IgG Antibody (Multiplex Flow Immunoassay); Endomysial IgA (ELISA)

Test Usage

The Celiac Disease Algorithm is utilized to streamline the serological testing for the diagnosis of celiac disease. The algorithm initially orders a serum IgA. Depending on the IgA result, further testing is reflexively ordered and processed. If the serum IgA is very low (<6 mg/dL), transglutaminase IgG AB (TTGG) and deamidated gliadin IgG AB (GLADG) are automatically performed. If the serum IgA low (>6, but below age matched reference range), transglutaminase IgG and IgA antibody tests (TTGG, TTGA), and deamidated gliadin IgG and IgA antibody tests (GLADG, GLADA) are reflexively performed. If the initial serum IgA result is normal or elevated, transglutaminase IgA AB (TTGA) is performed. If the transglutaminase IgA AB is negative (<15) or positive (>30), no further testing is performed. Testing is complete. If the transglutaminase IgA AB is weakly positive (15-30), endomysial IgA AB (EMA) and deamidated gliadin IgA AB (GLADA) testing are reflexively performed.

Reference Range *

Refer to individual test entries.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Monday - Friday (initial IgA test); refer to individual test entries for reflexive testing.
Analytic Time

8 hours (initial IgA test); refer to individual test entries for reflexive testing.

Soft Order Code
MiChart Code
Celiac Disease, Algorithm
  • Celiac Panel
Chemical Pathology
Specimen Requirements
Collection Instructions

Collect specimen in a red top or SST tube. Centrifuge, aliquot serum into a plastic vial and refrigerate for 7 days or freeze for longer storage.

Alternate Specimen
Red top tube
Normal Volume
1.5 mL serum
Minimum Volume
0.75 mL serum
Additional Information

By ordering this test the clinician acknowledges that additional reflex testing will be performed and billed at a separate additional charge if indicated.

CPT Code
Fee Code
Reflex CPT
GLADA 83516 GLADG 83516 TTGA 83516 TTGG 83516 EMA 86255
Reflex Fee Code
GLADA 35601 GLADG 35602 TTGA 35603 TTGG 35604 EMA 21947