Test Overview
Test Methodology

Crossed Immunoelectrophoresis

Test Usage

Useful in distinguishing between the types of membranoproliferative glomerulonephritis and for monitoring treatment.

Reference Range *

0.00 - 0.30

Test Details
Days Set Up
Tuesday
Analytic Time

14 - 16 days

Soft Order Code
MMLR
Synonyms
  • C3NeF
  • Complement C3 Nephritic Factor
Laboratory
Sendout
Reference Laboratory
Mayo Wild 85 (ZW85) (National Jewish Health)
Section
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a red top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and freeze within 2 hours of collection.

Rejection Criteria
SST tube not acceptable.
Yellow Top Tube
Red Top Tube
Normal Volume
1 mL serum
Minimum Volume
0.25 mL serum
Additional Information

C3 NeF is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo. Its presence can be detected by mixing the patient's serum with normal serum and looking for conversion of the C3 in the normal serum by 2-dimensional immunoelectrophoresis under conditions that only allow alternative pathway activation. This assay measures C3 NeF function and is semi-quantitative. Test sent to Mayo Medical Laboratories; performed by National Jewish Health.

Billing
CPT Code
86161
NY State Approved
No