Test Overview
Test Methodology

Crossed Immunoelectrophoresis

Test Usage

Useful in distinguishing between the types of membranoproliferative glomerulonephritis and for monitoring treatment.

Reference Range *

0.00 - 0.30

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details
Days Set Up
Analytic Time

14 - 16 days

Soft Order Code
  • C3NeF
  • Complement C3 Nephritic Factor
Reference Laboratory
Mayo Wild 85 (ZW85, C3NF) (National Jewish Health)
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in a red top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and freeze within 2 hours of collection.

Normal Volume
1 mL serum
Minimum Volume
0.25 mL serum
Rejection Criteria
SST tube not acceptable.
Additional Information

C3 NeF is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo. Its presence can be detected by mixing the patient's serum with normal serum and looking for conversion of the C3 in the normal serum by 2-dimensional immunoelectrophoresis under conditions that only allow alternative pathway activation. This assay measures C3 NeF function and is semi-quantitative. Test sent to Mayo Medical Laboratories; performed by National Jewish Health.

CPT Code