Test Overview

Test Methodology

Crossed Immunoelectrophoresis

Test Usage

Useful in distinguishing between the types of membranoproliferative glomerulonephritis and for monitoring treatment.

Reference Range*

0.00 - 0.30

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Details

Days Test Performed


Analytic Time

14 - 16 days

Soft Order Code




Complement C3 Nephritic Factor

+ See More



Laboratory Reference

Mayo Wild 85 (ZW85) (National Jewish Health)

Looking to Order a Test?

We’ve provided helpful links to make ordering easy.

Find a Requisition

All specimens should be accompanied by a requisition.

Submitting Specimens

Learn about how to properly label and where to ship specimens.

Order Kits and Supplies

MLabs provides all the supplies necessary for the collection of specimens.

Test FAQ

Visit our provider FAQ and learn about common questions to ordering tests.

Specimen Requirements

Offsite Collection Instructions

Collect specimen in a red top tube; do not use SST tube. Centrifuge, aliquot serum into a plastic vial and freeze within 2 hours of collection.

Rejection Criteria

SST tube not acceptable.


Yellow Top Tube
Red Top Tube

Normal Volume

1 mL serum

Minimum Volume

0.25 mL serum

Additional Information

C3 NeF is an autoantibody that binds to the alternative pathway C3 convertase and prolongs its activity in vivo. Its presence can be detected by mixing the patient's serum with normal serum and looking for conversion of the C3 in the normal serum by 2-dimensional immunoelectrophoresis under conditions that only allow alternative pathway activation. This assay measures C3 NeF function and is semi-quantitative. Test sent to Mayo Medical Laboratories; performed by National Jewish Health.

Billing Information

Fee Codes

CPT Code


Have More Billing Questions?

Explore our helpful billing resources:

Billing Resources

Billing FAQ