Test Overview
Test Methodology

Polymerase Chain Reaction (PCR) and sequencing

Test Usage

Broad Range (Universal) PCR is requested on fresh tissue or fluid when in-house cultures and smears yield negative results and the clinical team’s suspicion for infection remain.
There are multiple choices this test can include, most commonly Bacteria, Fungi and AFB. (or any combo thereof). Please be sure to indicate on the UW req which test(s) are desired

Reference Range *

Not Detected

Test Details
Days Set Up
Monday - Saturday
Analytic Time

7 - 10 days

Soft Order Code
MiChart Code
  • Universal PCR
Reference Laboratory
University of Washington Molecular Microbiology
Special Testing
Specimen Requirements

A separate specimen should be collected in a sterile container without formalin and labelled with “broad range PCR” on the sticker.

Clinician must order Broad Range PCR in MiChart. The on-service resident or covering director will receive an automated page once this order is placed for review. This pager is covered Monday-Friday, 8 am to 5 pm. Orders placed on off hours will be followed up the next week day.

All Broad range PCR must be approved before it is sent out. Clinician will be contacted by Microbiology attending and/or resident to discuss order during normal work hours. The Broad Range PCR send out may be held to confirm that routine cultures, or smears, are negative.

Special Handling

Specimen received for culture will not routinely have a portion set aside for Broad Range PCR unless a Broad Range PCR order has been placed in MiChart.

Rejection Criteria
Specimen received in formalin
Normal Volume
2.0 mL fluid or 1 cubic centimeter of tissue
Minimum Volume
0.5 mL or as much as possible tissue
Storage Temperature
Frozen for fresh tissue and fluids; blocks are sent ambient with a gel pack to prevent temperature extremes during travel
Additional Information

Test sent to University of Washington Molecular Microbiology laboratory

CPT Code
87801 x2, 87556
NY State Approved