Polymerase Chain Reaction (PCR) and sequencing
Broad Range (Universal) PCR is requested on fresh tissue or fluid when in-house cultures and smears yield negative results and the clinical team’s suspicion for infection remain.
There are multiple choices this test can include, most commonly Bacteria, Fungi and AFB. (or any combo thereof). Please be sure to indicate on the UW req which test(s) are desired. Standard Bacterial PCR is what should typically be marked. If the reflex to NGS is indicated, confirm with Micro administration that test is approved.
7 - 10 days
- Universal PCR
A separate specimen should be collected in a sterile container without formalin and labelled with “broad range PCR” on the sticker.
Clinician must order Broad Range PCR in MiChart. The on-service resident or covering director will receive an automated page once this order is placed for review. This pager is covered Monday-Friday, 8 am to 5 pm. Orders placed on off hours will be followed up the next week day.
All Broad range PCR must be approved before it is sent out. Clinician will be contacted by Microbiology attending and/or resident to discuss order during normal work hours. The Broad Range PCR send out may be held to confirm that routine cultures, or smears, are negative.
Standard Bacterial PCR is what should typically be marked. If the reflex to NGS is indicated, confirm with Micro administration that test is approved.
Specimen received for culture will not routinely have a portion set aside for Broad Range PCR unless a Broad Range PCR order has been placed in MiChart.
Test sent to University of Washington Molecular Microbiology laboratory