Label a glass slide with patient's last name, first name and registration number (or other second unique patient identifier) using a graphite pencil (do not use ink, wax or crayon). If smears are prepared from both breasts, also specify right or left.

Gently strip subareolar area and nipple with thumb and forefinger. When secretion occurs, allow pea sized drop to accumulate on apex of nipple. Place slide on nipple and then draw slide quickly across nipple. Fix slide IMMEDIATELY with spray fixative. Repeat procedure until discharge is depleted.

Allow spray-fixed slides to dry completely and place into a slide holder. Submit with an appropriately completed requisition, including pertinent patient history.