Test Overview
Test Methodology

Multiplex Polymerase Chain Reaction (PCR)

Test Usage

To detect genetic polymorphism between the patient (recipient) and the donor prior to the bone marrow transplant, in order to evaluate the engraftment status after the transplant.

Reference Range *

Interpretive report provided. A combined report for the recipient and donor is issued under the patient (recipient) medical record number; no separate report is issued for the donor.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

If the patient has a very low white blood cell count, the DNA yield may be insufficient to complete the analysis. In order to perform this test, both the patient's pre-transplant and the donor's specimen must be available. Specimens submitted for Molecular Diagnostics testing may contain substances that can inhibit PCR gene amplification. If an inhibitor is known to be present, attempts will be made to clear the specimen of inhibitor prior to amplification. Known inhibitors include heparin, medications and some levels of hemoglobin or IgG. The major inhibitor we see in our laboratory is heparin; avoid drawing blood in a heparin tube or through a heparinized port. For molecular diagnostics testing, a lavender top (EDTA) tube is preferred. If specimens containing heparin must be submitted, note "heparin" on the requisition to alert the lab to 'clean' the specimen before analysis thus reducing turnaround time, improving retention of DNA in the specimen and increasing our ability to return a conclusive result.

Test Details
Analytic Time

2 - 7 days

Soft Order Code
DONBM
MiChart Code
BMT Engraftment Analysis, Pre-BMT, Donor
Synonyms
  • Bone Marrow Transplant Engraftment Evaluation
  • Polymorphism Detection, pre-BMT
  • Pre-BMT Engraftment Analysis, Donor
  • Pre-BMT Polymorphism Detection
  • VNTR for pre-BMT Engraftment Analysis
  • MOL DX ADD
  • DONBM
  • Donor BM Engraph. Eval
  • CEDON
  • Chimerism Analysis, Pre-Transplant, Donor
  • MLABEL
  • BMT DONOR
Laboratory
Molecular Diagnostics
Section
Molecular Diagnostics
Specimen Requirements
Collection Instructions

Collect blood or bone marrow in lavender top tube. Send intact specimen at room temperature or refrigerated.

Alternate Specimen
The preferred specimen is EDTA (purple top); ACD (yellow top) is also acceptable. The assay will be attempted on Green top (sodium or lithium heparin) tubes, but the test may fail due to inhibition of PCR by heparin and an inadequate result will be issued in a report. Extracted DNA is acceptable. [rev 3/10; 8/15]
Lavender Top Tube
Normal Volume
6 mL EDTA whole blood; 3 mL EDTA bone marrow. Extracted DNA is also acceptable if extracted in a CLIA certified laboratory.
Minimum Volume
7 mL EDTA whole blood; 1.5 mL EDTA bone marrow
Additional Information

By ordering this test the clinician acknowledges that informed consent has been obtained from the patient as required by applicable state or federal laws and the ordering clinician has authorization from the patient permitting MLabs to report the test results to the ordering clinician. Test includes pathologist interpretation of results billed as a separate additional charge. This test is not available without interpretation.

Billing
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