Test Overview
Test Methodology

Polymerase Chain Reaction (PCR)

Test Usage

The QBKV assay is intended to measure BK viral load in patient plasma.

Reference Range *

Not detected.
Reportable Range: Log10 2.1 (126 copies/mL) to Log10 7.8 (62,800,000 copies/mL)
Virus that is detectable at levels above or below the reportable range will be reported but not quantified.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

A change of less than 0.5 log10 copies/mL from the previous value is within the expected measurement error of this assay.

Test Details
Days Set Up
Monday, Wednesday, Friday
Analytic Time

8 hours

Soft Order Code
QBKV
MiChart Code
BK Virus DNA, Quantitative, Plasma
Laboratory
Microbiology
Section
Microbiology
Specimen Requirements
Collection Instructions

Collect specimen in a 6 ml lavender top (EDTA) tube. Centrifuge for 20 minutes at 800-1600 xg at room temperature and aliquot plasma into a polypropylene plastic vial within 24 hours of collection. Refrigerate plasma at 2-8 C for up to 5 days or freeze at -70 C for up to 2 weeks. Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the viral load. Microbiology molecular testing requires a dedicated sample, and cannot be shared with other laboratories.

Normal Volume
4 mL whole blood or 2 mL EDTA plasma
Minimum Volume
2 mL whole blood or 1 mL EDTA plasma
Storage Temperature
Specimen can be held up to 24 hours prior to centrifugation at 2-8 C. Post centrifugation plasma aliquot may be held at 15- 30 C for up to 24 hours, held at 2-8 C for up to 5 days, or at -70 C for up to 2 weeks.
Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the viral load.
Billing
CPT Code
87799
Fee Code
LA019
LOINC
44805-0