Polymerase Chain Reaction (PCR)
The QBKV assay is intended to measure BK viral load in patient plasma.
Reportable Range: Log10 2.1 (126 copies/mL) to Log10 7.8 (62,800,000 copies/mL)
Virus that is detectable at levels above or below the reportable range will be reported but not quantified.
A change of less than 0.5 log10 copies/mL from the previous value is within the expected measurement error of this assay.
Collect specimen in a 6 ml lavender top (EDTA) tube. Centrifuge for 20 minutes at 800-1600 xg at room temperature and aliquot plasma into a polypropylene plastic vial within 24 hours of collection. Refrigerate plasma at 2-8 C for up to 5 days or freeze at -70 C for up to 2 weeks. Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the viral load. Microbiology molecular testing requires a dedicated sample, and cannot be shared with other laboratories.
Do not allow frozen specimens to thaw at any time during storage or transport, due to possible degradation of the viral load.