Test Overview
Test Methodology

Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)

Test Usage

Bile acids are elevated in individuals with liver dysfunction, such as hepatitis, cirrhosis, and intrahepatic cholestasis of pregnancy.

Reference Range *

Cholic Acid: < or = 5.0 nmol/mL; Chenodoxycholic Acid: < or = 6.0 nmol/mL; Deoxycholic Acid: < or = 6.0 nmol/mL; Ursodeoxycholic Acid: < or = 2.0 nmol/mL; Total Bile Acids: < or = 19.0 nmol/mL.

Test Limitations

Do not use for the characterization of peroxisomal biogenesis disorders or inborn errors of metabolism.

Test Details
Days Set Up
Monday - Friday
Analytic Time

2 - 7 days

Soft Order Code
  • Deoxycholic Acid
  • Cholic Acid
  • Chenodeoxycholic Acid
  • Bile Salts, Fractionated
Reference Laboratory
Mayo BAFS (62234)
Special Testing
Specimen Requirements
Collection Instructions

Collect specimen in red top tube from a fasting patient (overnight fast). Centrifuge, aliquot serum into a plastic vial and refrigerate (preferred) or freeze.

Special Handling

12-14 hr fasting specimen.

Normal Volume
0.5 mL serum
Minimum Volume
0.3 mL serum
Additional Information

Test sent to Mayo Medical Laboratories.

CPT Code
Fee Code