Test Overview

Test Methodology

Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)

Test Usage

Bile acids are elevated in individuals with liver dysfunction, such as hepatitis, cirrhosis, and intrahepatic cholestasis of pregnancy.

Reference Range*

Cholic Acid: < or = 5.0 nmol/mL; Chenodoxycholic Acid: < or = 6.0 nmol/mL; Deoxycholic Acid: < or = 6.0 nmol/mL; Ursodeoxycholic Acid: < or = 2.0 nmol/mL; Total Bile Acids: < or = 19.0 nmol/mL.

* Reference ranges may change over time. Please refer to the original patient report when evaluating results.

Test Limitations

Do not use for the characterization of peroxisomal biogenesis disorders or inborn errors of metabolism.

Test Details

Days Test Performed


Analytic Time

2 - 7 days

Soft Order Code




Deoxycholic Acid
Cholic Acid
Chenodeoxycholic Acid
Bile Salts, Fractionated

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Laboratory Reference

Mayo BAFS (62234)

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Specimen Requirements

Offsite Collection Instructions

Collect specimen in red top tube from a fasting patient (overnight fast). Centrifuge, aliquot serum into a plastic vial and refrigerate (preferred) or freeze.

Special Handing

Fasting specimen.


Red Top Tube

Normal Volume

0.5 mL serum

Minimum Volume

0.3 mL serum

Additional Information

Test sent to Mayo Medical Laboratories.

Billing Information

Fee Codes

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